Brachyury Antibody [NL557]

Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X 100, and 1% BSA. After blocking, sections were incubated with NL557 conjugated antibody at a final concentration of 1X (1:10 dilution) in blocking buffer for 3 hours at room temperature in the dark. Between each step, cells were washed with PBS containing BSA.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: NL557

Brachyury Antibody [NL557] Summary

• Immunogen: E. coli‑derived, recombinant human Brachyury
  Ser2-Glu202
  Accession # O15178
• Specificity: Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: T
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:10 dilution

Packaging, Storage & Formulations

• Storage: Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
  *Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [NL557]

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brachyury Antibody (NL2085R)(1)

Publication using NL2085R

• Wu, CE;Huang, HP;Ho, HN;Chen, MJ; Generation of a human induced pluripotent stem cell line NTUHi006-A from a polycystic ovarian syndrome patient Stem cell research 2024-12-30 [PMID: 39742630] (Immunocytochemistry, Human)

Bioinformatics

• Gene Symbol: T
• Uniprot:
  • O15178()