Brachyury Antibody [Unconjugated]

Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.

NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ChIP, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Brachyury Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Brachyury
  Ser2-Glu202
  Accession # O15178
• Specificity: Detects human Brachyury in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: T
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brachyury Antibody (AF2085)(132)

Publications using AF2085

• Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease Cell Stem Cell, 2023;30(3):264-282.e9. 2023 [PMID: 36868194] (Flow Cytometry, Human)
• L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 2022;66(0):102995. 2022-01-01 [PMID: 36528012] (ICC, Human)
• CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell, 2022;29(12):1685-1702.e22. 2022-01-01 [PMID: 36459969] (ICC, Human)
• DC Miller, P Lisowski, S Lickfett, B Mlody, M Bünning, C Genehr, C Ulrich, EE Wanker, S Diecke, J Priller, A Prigione Generation of induced pluripotent stem cells from three individuals with Huntington's disease Stem Cell Research, 2022;65(0):102976. 2022-01-01 [PMID: 36434993] (Flow Cytometry, Human)
• M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension Nature Cell Biology, 2022;24(9):1341-1349. 2022-01-01 [PMID: 36100738] (IHC, Mouse)
• K Chen, YA Martens, A Meneses, DH Ryu, W Lu, AC Raulin, F Li, J Zhao, Y Chen, Y Jin, C Linares, M Goodwin, Y Li, CC Liu, T Kanekiyo, DM Holtzman, TE Golde, G Bu, N Zhao LRP1 is a neuronal receptor for alpha-synuclein uptake and spread Molecular Neurodegeneration, 2022;17(1):57. 2022-01-01 [PMID: 36056345] (ICC, Human)
• G Cui, S Feng, Y Yan, L Wang, X He, X Li, Y Duan, J Chen, K Tang, P Zheng, PPL Tam, W Si, N Jing, G Peng Spatial molecular anatomy of germ layers in the gastrulating cynomolgus monkey embryo Cell Reports, 2022;40(9):111285. 2022-01-01 [PMID: 36044859] (ICC/IF, Cynomolgus Monkey)
• M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 2022;64(0):102892. 2022-01-01 [PMID: 35987121] (Flow Cytometry, Human)
• J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Stem Cell Research, 2022;64(0):102893. 2022-01-01 [PMID: 35987120] (Flow Cytometry, Human)
• Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation Stem Cell Research, 2022;64(0):102881. 2022-01-01 [PMID: 35944313] (Flow Cytometry, Human)
• J Liu, GC Black, SJ Kimber, PI Sergouniot Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing Stem Cell Research, 2022;64(0):102880. 2022-01-01 [PMID: 35933957] (Flow Cytometry, Human)
• S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion Oncogene, 2022;40(3):111119. 2022-01-01 [PMID: 35858555] (IHC, Human)
• YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1 Oncogene, 2022;63(0):102845. 2022-01-01 [PMID: 35728441] (ICC, Human)
• NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau A gastruloid model of the interaction between embryonic and extra-embryonic cell types Journal of tissue engineering, 2022;13(0):2041731422110. 2022-01-01 [PMID: 35707767] (IHC, Mouse)
• M Simunovic, ED Siggia, AH Brivanlou In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells Cell Stem Cell, 2022;29(6):962-972.e4. 2022-01-01 [PMID: 35659878] (IHC, Human)
• Y Shi, S Wu, B Yang, B Yu, Y Yan, J Yang, M Chu, X Hu Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus Stem Cell Research, 2022;62(0):102801. 2022-01-01 [PMID: 35609349] (ICC, Human)
• HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting Stem Cell Research, 2022;61(0):102779. 2022-01-01 [PMID: 35427904] (ICC, Human)
• A Manhas, JWS Jahng, CD Vera, SP Shenoy, JW Knowles, JC Wu Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants Stem Cell Research, 2022;61(0):102774. 2022-01-01 [PMID: 35413566] (ICC, Human)
• X Hu, S Xiong, X Zhou, L Sun Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587C�>�T in ADAT3 Stem Cell Research, 2022;61(0):102777. 2022-01-01 [PMID: 35405382] (ICC, Human)
• M Breyer, T Klein, K Klug, E Klopocki, N Üçeyler Generation of the induced pluripotent stem cell line UKWNLi005-A derived from a patient with the GLA mutation c.376A�>�G of unknown pathogenicity in Fabry disease Stem Cell Research, 2022;61(0):102747. 2022-01-01 [PMID: 35325818] (ICC, Human)
• W Chiu, A Li, T Wang, W Li, X Zhang Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology Stem Cell Research, 2022;60(0):102729. 2022-01-01 [PMID: 35247841] (ICC, Human)
• S Chumchuen, C Satirapod, A Tangprasit, S Hongeng Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts Stem Cell Research, 2022;60(0):102675. 2022-01-01 [PMID: 35114587] (ICC, Human)
• G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 2021;59(0):102635. 2021-01-01 [PMID: 35074682] (IHC, Human)
• N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met Stem Cell Research, 2021;59(0):102640. 2021-01-01 [PMID: 34971933] (Flow Cytometry, Human)
• K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg Stem Cell Research, 2021;59(0):102639. 2021-01-01 [PMID: 34971931] (Flow Cytometry, Human)
• C Lee, S Cho, C Lai, S Shenoy, R Vagelos, JC Wu Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant Stem Cell Research, 2021;59(0):102638. 2021-01-01 [PMID: 34954454] (Flow Cytometry, Human)
• N Belbachir, C Lai, JW Rhee, Y Zhuge, MV Perez, K Sallam, JC Wu Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations Stem Cell Research, 2021;57(0):102605. 2021-01-01 [PMID: 34856468] (Flow Cytometry, Human)
• B ?wierczek-, D Dudka, D Bauer, T Czajkowski, K Ilach, W Streminska, A Kominek, K Piwocka, MA Ciemerych, K Archacka Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions Cells, 2021;10(10):. 2021-01-01 [PMID: 34685722] (Immunolocalization, Mouse)
• C Labouesse, BX Tan, CC Agley, M Hofer, AK Winkel, GG Stirparo, HT Stuart, CM Verstreken, C Mulas, W Mansfield, P Bertone, K Franze, JCR Silva, KJ Chalut StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells Nature Communications, 2021;12(1):6132. 2021-01-01 [PMID: 34675200] (ICC, Mouse)
• L Scaramuzzi, V Lucchino, S Scalise, M Lo Conte, C Zannino, A Sacco, F Biamonte, EI Parrotta, FS Costanzo, G Cuda Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing Cells, 2021;10(9):. 2021-01-01 [PMID: 34572080] (ICC, Human)
• Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks Rapid target validation in a Cas9-inducible hiPSC derived kidney model Scientific Reports, 2021;11(1):16532. 2021-01-01 [PMID: 34400685] (IHC, Human)
• BC Liu, FY Liu, XY Gao, YL Chen, QQ Meng, YL Song, XH Li, SQ Bao Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells International Journal of Molecular Sciences, 2021;22(14):. 2021-01-01 [PMID: 34299091] (ICC, Human)
• C Guillot, Y Djeffal, A Michaut, B Rabe, O Pourquié Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo Elife, 2021;10(0):. 2021-01-01 [PMID: 34227938] (IHC, Chicken)
• G Iannello, A Patel, D Sirabella, B Corneo, V Thaker Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 2021;54(0):102432. 2021-01-01 [PMID: 34214899] (ICC, Human)
• L Ma, M Schmidt, EM Morrow Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls Stem Cell Research, 2021;54(0):102435. 2021-01-01 [PMID: 34182254] (ICC, Human)
• PS Huang, MH Wen, X Xie, A Xu, DF Lee, TY Chen Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1 Stem Cell Research, 2021;54(0):102415. 2021-01-01 [PMID: 34118566] (ICC, Human)
• MA Argenziano, M Burgos Ang, M Najari Bei, J Yang, AC Bertalovit, TV McDonald Generation of a heterozygous FLNC mutation-carrying human iPSC line, USFi002-A, for modeling dilated cardiomyopathy Stem Cell Research, 2021;53(0):102394. 2021-01-01 [PMID: 34088019] (ICC, Human)
• J Yang, E Samal, M Burgos Ang, A Bertalovit, TV McDonald Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA) Stem Cell Research, 2021;54(0):102398. 2021-01-01 [PMID: 34034221] (Flow Cytometry, Human)
• J Yang, M Burgos Ang, MA Argenziano, A Bertalovit, M Najari Bei, TV McDonald Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C) Stem Cell Research, 2021;54(0):102396. 2021-01-01 [PMID: 34029931] (Flow Cytometry, Human)
• DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah Induced pluripotent stem cells from subjects with Lesch-Nyhan disease Scientific Reports, 2021;11(1):8523. 2021-01-01 [PMID: 33875724] (ICC, Human)
• V Lucchino, L Scaramuzzi, S Scalise, K Grillone, M Lo Conte, C Esposito, U Aguglia, E Ferlazzo, N Perrotti, P Malatesta, EI Parrotta, G Cuda Generation of human induced pluripotent stem cell lines (UNIMGi003-A and UNIMGi004-A) from two Italian siblings affected by Unverricht-Lundborg disease Stem Cell Research, 2021;53(0):102329. 2021-01-01 [PMID: 33865103] (Flow Cytometry, Human)
• L Ma, AM Prada, M Schmidt, EM Morrow Generation of pathogenic TPP1 mutations in human stem cells as a model for neuronal ceroid lipofuscinosis type 2 disease Stem Cell Research, 2021;53(0):102323. 2021-01-01 [PMID: 33845243] (ICC, Human)
• LG Devito, L Healy, S Mohammed, F Guillemot, C Dias Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder Stem Cell Research, 2021;53(0):102304. 2021-01-01 [PMID: 33799280] (Flow Cytometry, Human)
• L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy Stem Cell Research, 2021;53(0):102279. 2021-01-01 [PMID: 33743363] (Flow Cytometry, Human)
• JWS Jahng, KE Black, L Liu, HR Bae, M Perez, EA Ashley, K Sallam, JC Wu Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene Stem Cell Research, 2021;53(0):102284. 2021-01-01 [PMID: 33743362] (Flow Cytometry, Human)
• B Schmid, B Holst, C Clausen, L Bahnassawy, P Reinhardt, MHM Bakker, E Díaz-Guerr, C Vicario, PV Martino-Ad, M Thoenes, A Ramirez, K Flissbach, C Grezella, O Brüstle, M Peitz, A Ebneth, A Cabrera-So Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (?2/?3/?4) and knock-out for the study of APOE biology in health and disease Stem Cell Research, 2021;52(0):102180. 2021-01-01 [PMID: 33556820] (ICC, Human)
• S Scalise, L Scaramuzzi, V Lucchino, C Esposito, P Malatesta, K Grillone, N Perrotti, G Cuda, EI Parrotta Generation of iPSC lines from two patients affected by febrile seizure due to inherited missense mutation in SCN1A gene Stem Cell Research, 2020;49(0):102083. 2020-01-01 [PMID: 33370868] (Flow Cytometry, Human)
• M Kinoshita, M Barber, W Mansfield, Y Cui, D Spindlow, GG Stirparo, S Dietmann, J Nichols, A Smith Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency Cell Stem Cell, 2020;0(0):. 2020-01-01 [PMID: 33271069] (Flow Cytometry, Mouse)
• FJ Hawkins, S Suzuki, ML Beermann, C Barillà, R Wang, C Villacorta, A Berical, JC Jean, J Le Suer, T Matte, C Simone-Roa, Y Tang, TM Schlaeger, AM Crane, N Matthias, SXL Huang, SH Randell, J Wu, JR Spence, G Carraro, BR Stripp, A Rab, EJ Sorsher, A Horani, SL Brody, BR Davis, DN Kotton Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells Cell Stem Cell, 2020;0(0):. 2020-01-01 [PMID: 33098807] (Flow Cytometry, Human)
• MA Jamil, H Singer, R Al-Rifai, N Nüsgen, M Rath, S Strauss, I Andreou, J Oldenburg, O El-Maarri Molecular Analysis of Fetal and Adult Primary Human Liver Sinusoidal Endothelial Cells: A Comparison to Other Endothelial Cells Int J Mol Sci, 2020;21(20):. 2020-01-01 [PMID: 33096636] (ICC, Human)
• SR Sripathy, Y Wang, RL Moses, A Fatemi, DA Batista, BJ Maher Generation of 10 patient-specific induced pluripotent stem cells (iPSCs) to model Pitt-Hopkins Syndrome Stem Cell Res, 2020;48(0):102001. 2020-01-01 [PMID: 32971458] (ICC, Human)
• PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA Stem Cell Res, 2020;48(0):101951. 2020-01-01 [PMID: 32798915] (ICC, Human)
• K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln Stem Cell Res, 2020;47(0):101895. 2020-01-01 [PMID: 32659731] (Flow Cytometry, Human)
• J Serra-Vina, MB Sandler, E Pak, W Zheng, A Dutra, W Introne, WA Gahl, MC Malicdan Generation and characterization of four Chediak-Higashi Syndrome (CHS) induced pluripotent stem cell (iPSC) lines Stem Cell Res, 2020;47(0):101883. 2020-01-01 [PMID: 32619719] (ICC, Human)
• I Buers, L Schöning, N Tomas Loge, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch Crisponi syndrome/cold-induced sweating syndrome type 2: Reprogramming of CS/CISS2 individual derived fibroblasts into three clones of one iPSC line Stem Cell Res, 2020;46(0):101855. 2020-01-01 [PMID: 32512309] (ICC, Human)
• L Schöning, NT Loges, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch, I Buers Generation of induced pluripotent stem cell lines from a Crisponi/Cold induced sweating syndrome type 1 individual Stem Cell Res, 2020;46(0):101820. 2020-01-01 [PMID: 32492556] (Flow Cytometry, Human)
• M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation Stem Cell Res, 2020;45(0):101781. 2020-01-01 [PMID: 32305865] (ICC, Human)
• A Gunne-Brad, A Sullivan, B Gharibi, RSM Sheriff, A Maity, YF Wang, A Edwards, M Jiang, M Howell, R Goldstone, R Wollman, P East, SDM Santos GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells Cell Stem Cell, 2020;26(5):693-706.e9. 2020-01-01 [PMID: 32302522] (ICC, Human)
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020;31(1):107471. 2020-01-01 [PMID: 32268087] (ICC, Human)
• M Paulis, L Susani, A Castelli, T Suzuki, T Hara, L Straniero, S Duga, D Strina, S Mantero, E Caldana, LS Sergi, A Villa, P Vezzoni Chromosome Transplantation: A Possible Approach to Treat Human X-linked Disorders Mol Ther Methods Clin Dev, 2020;17(0):369-377. 2020-01-01 [PMID: 32099849] (ICC, Human)
• P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val Stem Cell Res, 2020;43(0):101719. 2020-01-01 [PMID: 32062135] (ICC, Human)
• A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg Stem Cell Res, 2020;43(0):101720. 2020-01-01 [PMID: 32062131] (Flow Cytometry, Human)
• K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro Stem Cell Res, 2020;43(0):101714. 2020-01-01 [PMID: 32059175] (ICC, Human)
• P Limraksasi, T Kondo, M Zhang, H Okawa, T Osathanon, P Pavasant, H Egusa In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells Int J Mol Sci, 2020;21(2):. 2020-01-01 [PMID: 31963264] (IHC, Mouse)
• NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual Stem Cell Res, 2019;42(0):101679. 2019-01-01 [PMID: 31837633] (ICC, Human)
• Y Hu, CM Domínguez, J Bauer, S Weigel, A Schipperge, C Oelschlaeg, N Willenbach, S Keppler, M Bastmeyer, S Hei beta ler, C Wöll, T Scharnwebe, KS Rabe, CM Niemeyer Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings Nat Commun, 2019;10(1):5522. 2019-01-01 [PMID: 31797918] (ICC, Mouse)
• NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, V des Portes, D Germanaud, D Steinemann, G Göhring, R König Induced pluripotent stem cells (iPSCs) derived from a renpenning syndrome patient with c.459_462delAGAG mutation in PQBP1 (PEIi001-A) Stem Cell Res, 2019;41(0):101592. 2019-01-01 [PMID: 31698189] (ICC, Human)
• AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene Stem Cell Res, 2019;41(0):101595. 2019-01-01 [PMID: 31678773] (ICC, Human)
• P Wörsdörfer, N Dalda, A Kern, S Krüger, N Wagner, CK Kwok, E Henke, S Ergün Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells Sci Rep, 2019;9(1):15663. 2019-01-01 [PMID: 31666641] (Western Blot, Human)
• S Chhabra, L Liu, R Goh, X Kong, A Warmflash Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids PLoS Biol., 2019;17(10):e3000498. 2019-01-01 [PMID: 31613879] (ICC, Human)
• MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 2019;0(0):. 2019-01-01 [PMID: 31536674] (ICC, Human)
• J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 2019;40(0):101569. 2019-01-01 [PMID: 31520890] (ICC, Human)
• J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 2019;40(0):101570. 2019-01-01 [PMID: 31518904] (ICC, Human)
• S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019;39(0):101499. 2019-01-01 [PMID: 31352199] (ICC, Human)
• M Huang, J Tailor, Q Zhen, AH Gillmor, ML Miller, H Weishaupt, J Chen, T Zheng, EK Nash, LK McHenry, Z An, F Ye, Y Takashima, J Clarke, H Ayetey, FMG Cavalli, B Luu, BS Moriarity, S Ilkhanizad, L Chavez, C Yu, KM Kurian, T Magnaldo, N Sevenet, P Koch, SM Pollard, P Dirks, MP Snyder, DA Largaespad, YJ Cho, JJ Phillips, FJ Swartling, AS Morrissy, M Kool, SM Pfister, MD Taylor, A Smith, WA Weiss Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis Cell Stem Cell, 2019;0(0):. 2019-01-01 [PMID: 31204176] (IHC, Human)
• J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 2019;38(0):101468. 2019-01-01 [PMID: 31146251] (ICC, Human)
• T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019;0(0):. 2019-01-01 [PMID: 31031137] (IHC, Mouse)
• MC Regier, JJ Tokar, JW Warrick, L Pabon, E Berthier, DJ Beebe, KR Stevens User-defined morphogen patterning for directing human cell fate stratification Sci Rep, 2019;9(1):6433. 2019-01-01 [PMID: 31015521] (ICC, Human)
• X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells Sci Adv, 2019;5(3):eaau7887. 2019-01-01 [PMID: 30891497] (Western Blot, Human)
• M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 2019;36(0):101389. 2019-01-01 [PMID: 30798147] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 2019;35(0):101386. 2019-01-01 [PMID: 30685615] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations Stem Cell Res, 2019;35(0):101385. 2019-01-01 [PMID: 30685614] (ICC, Human)
• KE Worley, AS Chin, LQ Wan Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018;2018(0):1848605. 2018-01-01 [PMID: 30627170] (ICC, Human)
• JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene Stem Cell Res, 2018;34(0):101361. 2018-01-01 [PMID: 30611021] (ICC, Human)
• Baulier E, Garcia Diaz A, Corneo B, Farber D Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143. Stem Cell Res, 0;33(0):274-277. [PMID: 30513407]
• X Gao, JJ Yourick, RL Sprando Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 2018;31(0):193-196. 2018-01-01 [PMID: 30099336] (ICC, Human)
• AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL Stem Cell Res, 2018;31(0):157-160. 2018-01-01 [PMID: 30096712] (ICC, Human)
• SE Harrison, B Sozen, M Zernicka-G In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells Nat Protoc, 2018;0(0):. 2018-01-01 [PMID: 29988106] (ICC, Mouse)
• B López-Esco, JM Caro-Vega, DS Vijayragha, TF Plageman, JA Sanchez-Al, RC Moreno, D Savery, J Márquez-Ri, LA Davidson, P Ybot-Gonzá The non-canonical Wnt-PCP pathway shapes the caudal neural plate Development, 2018;0(0):. 2018-01-01 [PMID: 29636380] (IHC, Mouse)
• NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 2018;22(4):543-558.e12. 2018-01-01 [PMID: 29625069] (ICC, Mouse)
• S Schmidt, N Schumacher, J Schwarz, S Tangermann, L Kenner, M Schlederer, M Sibilia, M Linder, A Altendorf-, T Knösel, ES Gruber, G Oberhuber, J Bolik, A Rehman, A Sinha, J Lokau, P Arnold, AS Cabron, F Zunke, C Becker-Pau, A Preaudet, P Nguyen, J Huynh, S Afshar-Ste, AL Chand, J Westermann, PJ Dempsey, C Garbers, D Schmidt-Ar, P Rosenstiel, T Putoczki, M Ernst, S Rose-John ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling J. Exp. Med., 2018;0(0):. 2018-01-01 [PMID: 29472497] (IHC-P, Mouse)
• N Briand, AC Guénantin, D Jeziorowsk, A Shah, M Mantecon, E Capel, M Garcia, A Oldenburg, J Paulsen, JS Hulot, C Vigouroux, P Collas The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks Hum. Mol. Genet., 2018;0(0):. 2018-01-01 [PMID: 29438482] (Western Blot, Human)
• A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions Mol. Syst. Biol., 2018;14(1):e7952. 2018-01-01 [PMID: 29378814] (ICC, Mouse)
• MT De Angelis, EI Parrotta, G Santamaria, G Cuda Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells Cell Death Dis, 2018;9(1):6. 2018-01-01 [PMID: 29305588] (ICC, Human)
• S Martins, M Bohndorf, F Schröter, F Assar, W Wruck, K Sleegers, C Van Broeck, J Adjaye Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female Stem Cell Res, 2017;26(0):76-79. 2017-01-01 [PMID: 29268155] (ICC, Human)
• CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency Nat Commun, 2017;8(1):1149. 2017-01-01 [PMID: 29074849] (ICC, Human)
• J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening Sci Rep, 2017;7(1):8491. 2017-01-01 [PMID: 28819231] (ICC, Human)
• KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017;0(0):. 2017-01-01 [PMID: 28602613] (ICC, Human)
• MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich Guided self-organization and cortical plate formation in human brain organoids Nat. Biotechnol., 2017;0(0):. 2017-01-01 [PMID: 28562594] (IHC, Human)
• M Oginuma, P Moncuquet, F Xiong, E Karoly, J Chal, K Guevorkian, O Pourquié A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos Dev. Cell, 2017;40(4):342-353.e10. 2017-01-01 [PMID: 28245921] (IHC, Chicken)
• Joan Massagué The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells Cell Stem Cell, 2016;0(0):. 2016-01-01 [PMID: 27889317] (IHC, Mouse)
• Dong-An Wang Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor Acta Biomater, 2016;0(0):. 2016-01-01 [PMID: 27845273] (ICC, Mouse)
• Kiyokazu Agata Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos Dev. Biol., 2016;0(0):. 2016-01-01 [PMID: 27818184] (IHC-P, Mouse)
• Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells PLoS ONE, 2016;11(11):e0165715. 2016-01-01 [PMID: 27802323] (IHC-P, Human)
• ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016;17(2):222-227. 2016-01-01 [PMID: 27591478] (IHC, Human)
• Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells PLoS ONE, 2016;11(7):e0159246. 2016-01-01 [PMID: 27409080] (IHC-Fr, Mouse)
• L Song, J Chen, G Peng, K Tang, N Jing Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling J. Biol. Chem., 2016;291(29):15212-25. 2016-01-01 [PMID: 27226536] (ICC, Mouse)
• Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun, 2015;6(0):7212. 2015-01-01 [PMID: 25998442] (Western Blot, Human)
• Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J An alternative pluripotent state confers interspecies chimaeric competency. Nature, 2015;521(7552):316-21. 2015-01-01 [PMID: 25945737] (IHC, Human)
• Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Development, 2015;142(9):1628-38. 2015-01-01 [PMID: 25922526] (IHC-Fr, Mouse)
• Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2015;33(1):89-96. 2015-01-01 [PMID: 25383599] (IHC, Human)
• Gouti , Mina, Tsakiridis , Anestis, Wymeersch , Filip J, Huang , Yali, Kleinjung , Jens, Wilson , Valerie, Briscoe , James In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol, 2014;12(8):e1001937. 2014-01-01 [PMID: 25157815] (IHC, Human)
• Schwartz B, Marks M, Wittler L, Werber M, Wahrisch S, Nordheim A, Herrmann B, Grote P SRF is essential for mesodermal cell migration during elongation of the embryonic body axis. Mech Dev, 2014;133(0):23-35. 2014-01-01 [PMID: 25020278] (IHC-P, Mouse)
• Pinto F, Pertega-Gomes N, Pereira M, Vizcaino J, Monteiro P, Henrique R, Baltazar F, Andrade R, Reis R T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness. Clin Cancer Res, 2014;20(18):4949-61. 2014-01-01 [PMID: 25009296] (Western Blot, Human)
• Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. Nat Commun, 2014;5(0):3678. 2014-01-01 [PMID: 24759836] (IHC, Human)
• Tsakiridis , Anestis, Huang , Yali, Blin , Guillaum, Skylaki , Stavroul, Wymeersch , Filip, Osorno , Rodrigo, Economou , Costas, Karagianni , Eleni, Zhao , Suling, Lowell , Sally, Wilson , Valerie Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development, 2014;141(6):1209-21. 2014-01-01 [PMID: 24595287] (ICC, Mouse)
• Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm. Stem Cells, 2014;32(6):1493-502. 2014-01-01 [PMID: 24496962] (Flow Cytometry, Mouse)
• Fang F, Wasserman S, Torres-Vazquez J, Weinstein B, Cao F, Li Z, Wilson K, Yue W, Wu J, Xie X, Pei X The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function. J Cell Sci, 2014;127(0):1428-40. 2014-01-01 [PMID: 24463812] (IHC, Human)
• DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra P, Nagy A, van der Kooy D Oct4 is required ~E7.5 for proliferation in the primitive streak. PLoS Genet, 2013;9(11):e1003957. 2013-01-01 [PMID: 24244203] (IHC, Mouse)
• Evans AL, Faial T, Gilchrist MJ Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells. PLoS ONE, 2012;7(3):e33346. 2012-01-01 [PMID: 22479388] (ChIP, Human)
• Joubin , Katherin, Richardson , Amelia, Novoa , Natalia, Tu , Edmund, Tomishima , Mark J The endothelial cell line bEnd.3 maintains human pluripotent stem cells. Stem Cells Dev, 2012;21(12):2312-21. 2012-01-01 [PMID: 22224974] (Human)
• Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J. Cell. Sci., 2011;124(0):1992-2000. 2011-01-01 [PMID: 21610099] (ICC, Human)
• Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev. Biol., 2009;330(2):286-304. 2009-01-01 [PMID: 19358838] (ICC, Mouse)
• Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell, 2009;4(3):248-62. 2009-01-01 [PMID: 19265664] (ICC, Human)
• Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Nat. Cell Biol., 2008;10(7):837-48. 2008-01-01 [PMID: 18568018] (ICC, Human)
• Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood, 2007;111(4):1933-41. 2007-01-01 [PMID: 18042803] (ICC, Human)
• Thyagarajan B, Liu Y, Shin S, Lakshmipathy U, Scheyhing K, Xue H, Ellerstrom C, Strehl R, Hyllner J, Rao MS, Chesnut JD Creation of engineered human embryonic stem cell lines using phiC31 integrase. Stem Cells, 2007;26(1):119-26. 2007-01-01 [PMID: 17962703] (ICC, Human)
• Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R A porous photocurable elastomer for cell encapsulation and culture. Biomaterials, 2007;28(32):4826-35. 2007-01-01 [PMID: 17692371] (ICC, Human)
• De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Biotechnol. Bioeng., 2007;97(6):1617-25. 2007-01-01 [PMID: 17304565] (Western Blot, Human)
• McLean AB, D'Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007;25(1):29-38. 2007-01-01 [PMID: 17204604] (Western Blot, Human)
• Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Lett., 2006;580(25):5869-74. 2006-01-01 [PMID: 17027979] (ICC, Human)
• Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. 2006-01-01 [PMID: 16632596] (ICC, Human)

Reviews for Brachyury Antibody (AF2085) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous - IF Mouse 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24319666
• Species: Mouse

reviewed by: Anonymous - IF Human 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23658023
• Species: Human

Bioinformatics

• Gene Symbol: T
• Uniprot:
  • O15178()