Brachyury Antibody [Unconjugated]

Images

 
Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen ...read more
Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 ...read more
Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room ...read more
Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
View Available Formulations

Brachyury Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human TBX‑6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2085 in the following applications:

Publications
Read Publications using
AF2085 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46076
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
NB100-2737
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-47790
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-87441
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-27406
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2400
Species: Hu
Applications: WB, IHC, ChIP, ICC
AF644
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB600-717
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NBP2-57642
Species: Hu
Applications: WB, IHC, IHC-P
MAB6166
Species: Hu
Applications: WB, CyTOF-ready, ICC, ICFlow
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Brachyury Antibody (AF2085)(74)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 7 applications: ChIP, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(3)
ICC
(42)
IHC
(13)
IHC-Fr
(2)
IHC-P
(4)
Western Blot
(7)
All Applications
Filter By Species
Human
(53)
Mouse
(19)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using AF2085 Applications Species
K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln Stem Cell Res, 2020;47(0):101895. 2020 [PMID: 32659731] (Flow Cytometry, Human) Flow Cytometry Human
J Serra-Vina, MB Sandler, E Pak, W Zheng, A Dutra, W Introne, WA Gahl, MC Malicdan Generation and characterization of four Chediak-Higashi Syndrome (CHS) induced pluripotent stem cell (iPSC) lines Stem Cell Res, 2020;47(0):101883. 2020 [PMID: 32619719] (ICC, Human) ICC Human
L Schöning, NT Loges, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch, I Buers Generation of induced pluripotent stem cell lines from a Crisponi/Cold induced sweating syndrome type 1 individual Stem Cell Res, 2020;46(0):101820. 2020 [PMID: 32492556] (Flow Cytometry, Human) Flow Cytometry Human
M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation Stem Cell Res, 2020;45(0):101781. 2020 [PMID: 32305865] (ICC, Human) ICC Human
A Gunne-Brad, A Sullivan, B Gharibi, RSM Sheriff, A Maity, YF Wang, A Edwards, M Jiang, M Howell, R Goldstone, R Wollman, P East, SDM Santos GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells Cell Stem Cell, 2020;26(5):693-706.e9. 2020 [PMID: 32302522] (ICC, Human) ICC Human
AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020;31(1):107471. 2020 [PMID: 32268087] (ICC, Human) ICC Human
P Limraksasi, T Kondo, M Zhang, H Okawa, T Osathanon, P Pavasant, H Egusa In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963264] (IHC, Mouse) IHC Mouse
NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual Stem Cell Res, 2019;42(0):101679. 2019 [PMID: 31837633] (ICC, Human) ICC Human
Y Hu, CM Domínguez, J Bauer, S Weigel, A Schipperge, C Oelschlaeg, N Willenbach, S Keppler, M Bastmeyer, S Hei beta ler, C Wöll, T Scharnwebe, KS Rabe, CM Niemeyer Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings Nat Commun, 2019;10(1):5522. 2019 [PMID: 31797918] (ICC, Mouse) ICC Mouse
NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, V des Portes, D Germanaud, D Steinemann, G Göhring, R König Induced pluripotent stem cells (iPSCs) derived from a renpenning syndrome patient with c.459_462delAGAG mutation in PQBP1 (PEIi001-A) Stem Cell Res, 2019;41(0):101592. 2019 [PMID: 31698189] (ICC, Human) ICC Human
Show All 74 Publications.

Reviews for Brachyury Antibody (AF2085) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF2085:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
IF Mouse 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24319666
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24319666

Details

Detection NotesSee PMID 24319666

Comments

CommentsPublished: See PMID 24319666
  3
reviewed by:
Anonymous
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsPublished: See PMID 23658023

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Brachyury Antibody (AF2085) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 IC2085G
Allophycocyanin IC2085A
Biotin BAF2085
NL557 NL2085R
Phycoerythrin IC2085P

Additional Brachyury Products

Bioinformatics Tool for Brachyury Antibody (AF2085)

Discover related pathways, diseases and genes to Brachyury Antibody (AF2085). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Brachyury

There are no specific blogs for Brachyury, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

 
Anonymous
01/06/2015
Application: IF
Species: Mouse

 
Anonymous
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol T
Uniprot