Brachyury Antibody [Unconjugated]

Images

 
Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 ...read more
Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen ...read more
Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room ...read more
Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Brachyury Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) TBX-6, rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2085 in the following applications:

Publications
Read Publications using
AF2085 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (AF2085)(62)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 7 applications: ChIP, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(1)
ICC
(34)
IHC
(12)
IHC-Fr
(2)
IHC-P
(4)
Western Blot
(6)
All Applications
Filter By Species
Human
(43)
Mouse
(17)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using AF2085 Applications Species
NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual Stem Cell Res, 2019;42(0):101679. 2019 [PMID: 31837633] (ICC, Human) ICC Human
MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31536674] (ICC, Human) ICC Human
J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 2019;40(0):101569. 2019 [PMID: 31520890] (ICC, Human) ICC Human
J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 2019;40(0):101570. 2019 [PMID: 31518904] (ICC, Human) ICC Human
S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019;39(0):101499. 2019 [PMID: 31352199] (ICC, Human) ICC Human
J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 2019;38(0):101468. 2019 [PMID: 31146251] (ICC, Human) ICC Human
T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31031137] (IHC, Mouse) IHC Mouse
MC Regier, JJ Tokar, JW Warrick, L Pabon, E Berthier, DJ Beebe, KR Stevens User-defined morphogen patterning for directing human cell fate stratification Sci Rep, 2019;9(1):6433. 2019 [PMID: 31015521] (ICC, Human) ICC Human
X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells Sci Adv, 2019;5(3):eaau7887. 2019 [PMID: 30891497] (Western Blot, Human) Western Blot Human
M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 2019;36(0):101389. 2019 [PMID: 30798147] (ICC, Human) ICC Human
Show All 62 Publications.

Reviews for Brachyury Antibody (AF2085) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF2085:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24319666
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24319666

Details

Detection NotesSee PMID 24319666

Comments

CommentsPublished: See PMID 24319666
 
reviewed by:
Anonymous
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsPublished: See PMID 23658023

Product General Protocols

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FAQs for Brachyury Antibody (AF2085) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 IC2085G
Allophycocyanin IC2085A
Biotin BAF2085
NL557 NL2085R
Phycoerythrin IC2085P

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Bioinformatics Tool for Brachyury Antibody (AF2085)

Discover related pathways, diseases and genes to Brachyury Antibody (AF2085). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Brachyury

There are no specific blogs for Brachyury, but you can read our latest blog posts.
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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

 
Anonymous
01/06/2015
Application: IF
Species: Mouse

 
Anonymous
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol T
Uniprot
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