Brachyury Antibody [Unconjugated]

Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.

Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Brachyury Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Brachyury
  Ser2-Glu202
  Accession # O15178
• Specificity: Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human TBX‑6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: T
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brachyury Antibody (AF2085)(74)

Publications using AF2085

• K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln Stem Cell Res, 2020;47(0):101895. 2020 [PMID: 32659731] (Flow Cytometry, Human)
• J Serra-Vina, MB Sandler, E Pak, W Zheng, A Dutra, W Introne, WA Gahl, MC Malicdan Generation and characterization of four Chediak-Higashi Syndrome (CHS) induced pluripotent stem cell (iPSC) lines Stem Cell Res, 2020;47(0):101883. 2020 [PMID: 32619719] (ICC, Human)
• L Schöning, NT Loges, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch, I Buers Generation of induced pluripotent stem cell lines from a Crisponi/Cold induced sweating syndrome type 1 individual Stem Cell Res, 2020;46(0):101820. 2020 [PMID: 32492556] (Flow Cytometry, Human)
• M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation Stem Cell Res, 2020;45(0):101781. 2020 [PMID: 32305865] (ICC, Human)
• A Gunne-Brad, A Sullivan, B Gharibi, RSM Sheriff, A Maity, YF Wang, A Edwards, M Jiang, M Howell, R Goldstone, R Wollman, P East, SDM Santos GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells Cell Stem Cell, 2020;26(5):693-706.e9. 2020 [PMID: 32302522] (ICC, Human)
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020;31(1):107471. 2020 [PMID: 32268087] (ICC, Human)
• P Limraksasi, T Kondo, M Zhang, H Okawa, T Osathanon, P Pavasant, H Egusa In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963264] (IHC, Mouse)
• NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual Stem Cell Res, 2019;42(0):101679. 2019 [PMID: 31837633] (ICC, Human)
• Y Hu, CM Domínguez, J Bauer, S Weigel, A Schipperge, C Oelschlaeg, N Willenbach, S Keppler, M Bastmeyer, S Hei beta ler, C Wöll, T Scharnwebe, KS Rabe, CM Niemeyer Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings Nat Commun, 2019;10(1):5522. 2019 [PMID: 31797918] (ICC, Mouse)
• NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, V des Portes, D Germanaud, D Steinemann, G Göhring, R König Induced pluripotent stem cells (iPSCs) derived from a renpenning syndrome patient with c.459_462delAGAG mutation in PQBP1 (PEIi001-A) Stem Cell Res, 2019;41(0):101592. 2019 [PMID: 31698189] (ICC, Human)
• AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene Stem Cell Res, 2019;41(0):101595. 2019 [PMID: 31678773] (ICC, Human)
• P Wörsdörfer, N Dalda, A Kern, S Krüger, N Wagner, CK Kwok, E Henke, S Ergün Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells Sci Rep, 2019;9(1):15663. 2019 [PMID: 31666641] (Western Blot, Human)
• S Chhabra, L Liu, R Goh, X Kong, A Warmflash Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids PLoS Biol., 2019;17(10):e3000498. 2019 [PMID: 31613879] (ICC, Human)
• MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31536674] (ICC, Human)
• J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 2019;40(0):101569. 2019 [PMID: 31520890] (ICC, Human)
• J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 2019;40(0):101570. 2019 [PMID: 31518904] (ICC, Human)
• S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019;39(0):101499. 2019 [PMID: 31352199] (ICC, Human)
• J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 2019;38(0):101468. 2019 [PMID: 31146251] (ICC, Human)
• T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31031137] (IHC, Mouse)
• MC Regier, JJ Tokar, JW Warrick, L Pabon, E Berthier, DJ Beebe, KR Stevens User-defined morphogen patterning for directing human cell fate stratification Sci Rep, 2019;9(1):6433. 2019 [PMID: 31015521] (ICC, Human)
• X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells Sci Adv, 2019;5(3):eaau7887. 2019 [PMID: 30891497] (Western Blot, Human)
• M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 2019;36(0):101389. 2019 [PMID: 30798147] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 2019;35(0):101386. 2019 [PMID: 30685615] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations Stem Cell Res, 2019;35(0):101385. 2019 [PMID: 30685614] (ICC, Human)
• KE Worley, AS Chin, LQ Wan Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018;2018(0):1848605. 2018 [PMID: 30627170] (ICC, Human)
• JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene Stem Cell Res, 2018;34(0):101361. 2018 [PMID: 30611021] (ICC, Human)
• Baulier E, Garcia Diaz A, Corneo B, Farber D Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143. Stem Cell Res, 0;33(0):274-277. 0 [PMID: 30513407]
• X Gao, JJ Yourick, RL Sprando Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 2018;31(0):193-196. 2018 [PMID: 30099336] (ICC, Human)
• AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL Stem Cell Res, 2018;31(0):157-160. 2018 [PMID: 30096712] (ICC, Human)
• SE Harrison, B Sozen, M Zernicka-G In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells Nat Protoc, 2018;0(0):. 2018 [PMID: 29988106] (ICC, Mouse)
• B López-Esco, JM Caro-Vega, DS Vijayragha, TF Plageman, JA Sanchez-Al, RC Moreno, D Savery, J Márquez-Ri, LA Davidson, P Ybot-Gonzá The non-canonical Wnt-PCP pathway shapes the caudal neural plate Development, 2018;0(0):. 2018 [PMID: 29636380] (IHC, Mouse)
• NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 2018;22(4):543-558.e12. 2018 [PMID: 29625069] (ICC, Mouse)
• S Schmidt, N Schumacher, J Schwarz, S Tangermann, L Kenner, M Schlederer, M Sibilia, M Linder, A Altendorf-, T Knösel, ES Gruber, G Oberhuber, J Bolik, A Rehman, A Sinha, J Lokau, P Arnold, AS Cabron, F Zunke, C Becker-Pau, A Preaudet, P Nguyen, J Huynh, S Afshar-Ste, AL Chand, J Westermann, PJ Dempsey, C Garbers, D Schmidt-Ar, P Rosenstiel, T Putoczki, M Ernst, S Rose-John ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling J. Exp. Med., 2018;0(0):. 2018 [PMID: 29472497] (IHC-P, Mouse)
• N Briand, AC Guénantin, D Jeziorowsk, A Shah, M Mantecon, E Capel, M Garcia, A Oldenburg, J Paulsen, JS Hulot, C Vigouroux, P Collas The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks Hum. Mol. Genet., 2018;0(0):. 2018 [PMID: 29438482] (Western Blot, Human)
• A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions Mol. Syst. Biol., 2018;14(1):e7952. 2018 [PMID: 29378814] (ICC, Mouse)
• MT De Angelis, EI Parrotta, G Santamaria, G Cuda Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells Cell Death Dis, 2018;9(1):6. 2018 [PMID: 29305588] (ICC, Human)
• S Martins, M Bohndorf, F Schröter, F Assar, W Wruck, K Sleegers, C Van Broeck, J Adjaye Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female Stem Cell Res, 2017;26(0):76-79. 2017 [PMID: 29268155] (ICC, Human)
• CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency Nat Commun, 2017;8(1):1149. 2017 [PMID: 29074849] (ICC, Human)
• J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening Sci Rep, 2017;7(1):8491. 2017 [PMID: 28819231] (ICC, Human)
• KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28602613] (ICC, Human)
• MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich Guided self-organization and cortical plate formation in human brain organoids Nat. Biotechnol., 2017;0(0):. 2017 [PMID: 28562594] (IHC, Human)
• M Oginuma, P Moncuquet, F Xiong, E Karoly, J Chal, K Guevorkian, O Pourquié A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos Dev. Cell, 2017;40(4):342-353.e10. 2017 [PMID: 28245921] (IHC, Chicken)
• Joan Massagué The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells Cell Stem Cell, 2016;0(0):. 2016 [PMID: 27889317] (IHC, Mouse)
• Dong-An Wang Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor Acta Biomater, 2016;0(0):. 2016 [PMID: 27845273] (ICC, Mouse)
• Kiyokazu Agata Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos Dev. Biol., 2016;0(0):. 2016 [PMID: 27818184] (IHC-P, Mouse)
• Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells PLoS ONE, 2016;11(11):e0165715. 2016 [PMID: 27802323] (IHC-P, Human)
• ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016;17(2):222-227. 2016 [PMID: 27591478] (IHC, Human)
• Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells PLoS ONE, 2016;11(7):e0159246. 2016 [PMID: 27409080] (IHC-Fr, Mouse)
• L Song, J Chen, G Peng, K Tang, N Jing Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling J. Biol. Chem., 2016;291(29):15212-25. 2016 [PMID: 27226536] (ICC, Mouse)
• Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun, 2015;6(0):7212. 2015 [PMID: 25998442] (Western Blot, Human)
• Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J An alternative pluripotent state confers interspecies chimaeric competency. Nature, 2015;521(7552):316-21. 2015 [PMID: 25945737] (IHC, Human)
• Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Development, 2015;142(9):1628-38. 2015 [PMID: 25922526] (IHC-Fr, Mouse)
• Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2015;33(1):89-96. 2015 [PMID: 25383599] (IHC, Human)
• Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol, 2014;12(8):e1001937. 2014 [PMID: 25157815] (IHC, Human)
• Schwartz B, Marks M, Wittler L, Werber M, Wahrisch S, Nordheim A, Herrmann B, Grote P SRF is essential for mesodermal cell migration during elongation of the embryonic body axis. Mech Dev, 2014;133(0):23-35. 2014 [PMID: 25020278] (IHC-P, Mouse)
• Pinto F, Pertega-Gomes N, Pereira M, Vizcaino J, Monteiro P, Henrique R, Baltazar F, Andrade R, Reis R T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness. Clin Cancer Res, 2014;20(18):4949-61. 2014 [PMID: 25009296] (Western Blot, Human)
• Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. Nat Commun, 2014;5(0):3678. 2014 [PMID: 24759836] (IHC, Human)
• Tsakiridis, Anestis, Huang, Yali, Blin, Guillaum, Skylaki, Stavroul, Wymeersch, Filip, Osorno, Rodrigo, Economou, Costas, Karagianni, Eleni, Zhao, Suling, Lowell, Sally, Wilson, Valerie Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development, 2014;141(6):1209-21. 2014 [PMID: 24595287] (ICC, Mouse)
• Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm. Stem Cells, 2014;32(6):1493-502. 2014 [PMID: 24496962] (Flow Cytometry, Mouse)
• Fang F, Wasserman S, Torres-Vazquez J, Weinstein B, Cao F, Li Z, Wilson K, Yue W, Wu J, Xie X, Pei X The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function. J Cell Sci, 2014;127(0):1428-40. 2014 [PMID: 24463812] (IHC, Human)
• DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra P, Nagy A, van der Kooy D Oct4 is required ~E7.5 for proliferation in the primitive streak. PLoS Genet, 2013;9(11):e1003957. 2013 [PMID: 24244203] (IHC, Mouse)
• Evans AL, Faial T, Gilchrist MJ Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells. PLoS ONE, 2012;7(3):e33346. 2012 [PMID: 22479388] (ChIP, Human)
• Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J The endothelial cell line bEnd.3 maintains human pluripotent stem cells. Stem Cells Dev, 2012;21(12):2312-21. 2012 [PMID: 22224974] (Human)
• Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J. Cell. Sci., 2011;124(0):1992-2000. 2011 [PMID: 21610099] (ICC, Human)
• Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev. Biol., 2009;330(2):286-304. 2009 [PMID: 19358838] (ICC, Mouse)
• Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell, 2009;4(3):248-62. 2009 [PMID: 19265664] (ICC, Human)
• Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Nat. Cell Biol., 2008;10(7):837-48. 2008 [PMID: 18568018] (ICC, Human)
• Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood, 2007;111(4):1933-41. 2007 [PMID: 18042803] (ICC, Human)
• Thyagarajan B, Liu Y, Shin S, Lakshmipathy U, Scheyhing K, Xue H, Ellerstrom C, Strehl R, Hyllner J, Rao MS, Chesnut JD Creation of engineered human embryonic stem cell lines using phiC31 integrase. Stem Cells, 2007;26(1):119-26. 2007 [PMID: 17962703] (ICC, Human)
• Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R A porous photocurable elastomer for cell encapsulation and culture. Biomaterials, 2007;28(32):4826-35. 2007 [PMID: 17692371] (ICC, Human)
• De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Biotechnol. Bioeng., 2007;97(6):1617-25. 2007 [PMID: 17304565] (Western Blot, Human)
• McLean AB, D'Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007;25(1):29-38. 2007 [PMID: 17204604] (Western Blot, Human)
• Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Lett., 2006;580(25):5869-74. 2006 [PMID: 17027979] (ICC, Human)
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Reviews for Brachyury Antibody (AF2085) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous IF Mouse 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24319666
• Species: Mouse

Blocking

• Blocking Details: See PMID 24319666

Details

• Detection Notes: See PMID 24319666

Comments

• Comments: Published: See PMID 24319666

reviewed by: Anonymous IF Human 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23658023
• Species: Human

Blocking

• Blocking Details: See PMID 23658023

Details

• Detection Notes: See PMID 23658023

Comments

• Comments: Published: See PMID 23658023

Bioinformatics

• Gene Symbol: T
• Uniprot:
  • O15178()