Brachyury Antibody [Unconjugated]

Images

 
Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen ...read more
Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room ...read more
Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 ...read more
Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 ...read more
Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ChIP, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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View Available Formulations

Brachyury Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1-1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2085 in the following applications:

Publications
Read Publications using
AF2085 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (AF2085)(132)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Cynomolgus Monkey.

We have publications tested in 9 applications: ChIP, Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunolocalization, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(23)
ICC
(70)
ICC/IF
(1)
IHC
(21)
IHC-Fr
(2)
IHC-P
(4)
Immunolocalization
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(104)
Mouse
(24)
Chicken
(2)
Cynomolgus Monkey
(1)
All Species
Showing Publications 1 - 10 of 132. Show All 132 Publications.
Publications using AF2085 Applications Species
Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease Cell Stem Cell, 2023;30(3):264-282.e9. 2023 [PMID: 36868194] (Flow Cytometry, Human) Flow Cytometry Human
L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 2022;66(0):102995. 2022-01-01 [PMID: 36528012] (ICC, Human) ICC Human
CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell, 2022;29(12):1685-1702.e22. 2022-01-01 [PMID: 36459969] (ICC, Human) ICC Human
DC Miller, P Lisowski, S Lickfett, B Mlody, M Bünning, C Genehr, C Ulrich, EE Wanker, S Diecke, J Priller, A Prigione Generation of induced pluripotent stem cells from three individuals with Huntington's disease Stem Cell Research, 2022;65(0):102976. 2022-01-01 [PMID: 36434993] (Flow Cytometry, Human) Flow Cytometry Human
M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension Nature Cell Biology, 2022;24(9):1341-1349. 2022-01-01 [PMID: 36100738] (IHC, Mouse) IHC Mouse
K Chen, YA Martens, A Meneses, DH Ryu, W Lu, AC Raulin, F Li, J Zhao, Y Chen, Y Jin, C Linares, M Goodwin, Y Li, CC Liu, T Kanekiyo, DM Holtzman, TE Golde, G Bu, N Zhao LRP1 is a neuronal receptor for alpha-synuclein uptake and spread Molecular Neurodegeneration, 2022;17(1):57. 2022-01-01 [PMID: 36056345] (ICC, Human) ICC Human
G Cui, S Feng, Y Yan, L Wang, X He, X Li, Y Duan, J Chen, K Tang, P Zheng, PPL Tam, W Si, N Jing, G Peng Spatial molecular anatomy of germ layers in the gastrulating cynomolgus monkey embryo Cell Reports, 2022;40(9):111285. 2022-01-01 [PMID: 36044859] (ICC/IF, Cynomolgus Monkey) ICC/IF Cynomolgus Monkey
M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 2022;64(0):102892. 2022-01-01 [PMID: 35987121] (Flow Cytometry, Human) Flow Cytometry Human
J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Stem Cell Research, 2022;64(0):102893. 2022-01-01 [PMID: 35987120] (Flow Cytometry, Human) Flow Cytometry Human
Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation Stem Cell Research, 2022;64(0):102881. 2022-01-01 [PMID: 35944313] (Flow Cytometry, Human) Flow Cytometry Human
Show All 132 Publications.

Reviews for Brachyury Antibody (AF2085) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF2085:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
IF Mouse 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24319666
SpeciesMouse
  3
reviewed by:
Anonymous -
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Product General Protocols

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FAQs for Brachyury Antibody (AF2085) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

 
Anonymous -
01/06/2015
Application: IF
Species: Mouse

 
Anonymous -
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol T
Uniprot