BPGM Antibody (2E11) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

BPGM Antibody (2E11) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Recombinant human BPGM (1-259aa) purified from E. coli.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPGM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for BPGM Antibody (2E11) [CoraFluor™ 1]

• 2,3-bisphosphoglycerate mutase
• bisphosphoglycerate mutase
• BPG-dependent PGAM
• EC 3.1.3.132,3-bisphosphoglycerate synthase
• EC 5.4.2.1
• EC 5.4.2.4
• erythrocyte 2,3-bisphosphoglycerate mutase2,3-bisphosphoglycerate mutase, erythrocyte

Background

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds toand decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPGsynthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also hasphosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen.Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein,have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BPGM