BPGM Antibody (2E11) - BSA Free

Western Blot: BPGM Antibody (2E11) [NBP2-59493] - Lane 1: 293T cell lysates, Lane 2: BPGM transfected 293T cell lysates

Immunocytochemistry/Immunofluorescence: BPGM Antibody (2E11) [NBP2-59493] - Analysis of BPGM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BPGM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: BPGM Antibody (2E11) [NBP2-59493] - Analysis of BPGM in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BPGM Antibody (2E11) - BSA Free Summary

• Description: Novus Biologicals Mouse BPGM Antibody (2E11) - BSA Free (NBP2-59493) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human BPGM (1-259aa) purified from E. coli.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPGM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BPGM Antibody (2E11) - BSA Free

• 2,3-bisphosphoglycerate mutase
• bisphosphoglycerate mutase
• BPG-dependent PGAM
• EC 3.1.3.132,3-bisphosphoglycerate synthase
• EC 5.4.2.1
• EC 5.4.2.4
• erythrocyte 2,3-bisphosphoglycerate mutase2,3-bisphosphoglycerate mutase, erythrocyte

Background

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds toand decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPGsynthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also hasphosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen.Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein,have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BPGM