BP1 Antibody (1F11) - Azide and BSA Free

Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11.Lane 1: DLX4 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.

Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi ( Cat # H00001748-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DLX4 monoclonal antibody (M01), clone 1F11 (Cat # H00001748-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 transfected lysate using anti-DLX4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DLX4 MaxPab rabbit polyclonal antibody.

ELISA: BP1 Antibody (1F11) [H00001748-M01] - Detection limit for recombinant GST tagged DLX4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, KD
• Clone: 1F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BP1 Antibody (1F11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DLX4 (AAH16145, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTSLPCPLPGRDASKAVFPDLAPVPSVAAAYPLGLSPTTAASPNLSYSRPYGHLLSYPYTEPANPGDSYLSCQQPAALSQPLCGPAEHPQELEADSEK
• Specificity: DLX4 - distal-less homeobox 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLX4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BP1 Antibody (1F11) - Azide and BSA Free

• Beta protein 1
• BP1distal-less homeo box 7
• distal-less homeobox 4
• DLX7distal-less homeo box 9
• DLX8DLX9
• homeobox protein DLX-4
• Homeobox protein DLX-7
• Homeobox protein DLX-8

Background

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. Three transcript variants have been described for this gene, however, the full length nature of one variant has not been described. Studies of the two splice variants revealed that one encoded isoform functions as a repressor of the beta-globin gene while the other isoform lacks that function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BP1 Antibody (H00001748-M01)(3)

Publications using H00001748-M01

• Wu D, Mandal S, Choi A et al. DLX4 is associated with orofacial clefting and abnormal jaw development. Hum Mol Genet. 2015-05-07 [PMID: 25954033]
• Trinh BQ, Barengo N, Naora H. Homeodomain protein DLX4 counteracts key transcriptional control mechanisms of the TGF-?] cytostatic program and blocks the antiproliferative effect of TGF-?]. Oncogene. 2011-02-07 [PMID: 21297662]
• Hara F, Samuel S, Liu J et al. A homeobox gene related to Drosophila distal-less promotes ovarian tumorigenicity by inducing expression of vascular endothelial growth factor fibroblast growth factor-2. Am J Pathol;170(5):1594-606. 2007-05-01 [PMID: 17456765]

Bioinformatics

• Gene Symbol: DLX4
• Entrez:
  • 1748(Human)
• OMIM:
  • 601911(Human)
• Uniprot:
  • AAH16145(Human)