BORIS Antibody (2B10) - BSA Free

Western Blot: BORIS Antibody (2B10) [NBP3-42935] - HeLa cells were subjected to SDS PAGE followed by western blot with (BORIS antibody) at dilution of 1:1000

Immunocytochemistry/ Immunofluorescence: BORIS Antibody (2B10) [NBP3-42935] - Immunofluorescent analysis of MCF-7 cells, using (BORIS antibody) at 1:25 dilution andRhodamine-labeled goat anti-mouse IgG (red).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

BORIS Antibody (2B10) - BSA Free Summary

• Description: Novus Biologicals Mouse BORIS Antibody (2B10) - BSA Free (NBP3-42935) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CCCTC-binding factor(zinc finger protein)-like - (Uniprot# Q8NI51)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTCFL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for BORIS Antibody (2B10) - BSA Free

• BORISHMGB1L1
• BORIS-like protein
• Brother of the regulator of imprinted sites
• Cancer/testis antigen 27
• CCCTC-binding factor (zinc finger protein)-like
• CCCTC-binding factor
• CT27MGC163358
• CTCF paralog
• CTCF-like protein
• CTCF-T
• dJ579F20.2
• HMG-1L1
• MGC169105
• MGC169106
• putative high mobility group protein 1-like 1
• putative high mobility group protein B1-like 1
• transcriptional repressor CTCFL
• Zinc finger protein CTCF-T

Background

BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTCFL