Borealin Antibody (OTI4G3)

Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.

Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI4G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Borealin Antibody (OTI4G3) Summary

• Description: Novus Biologicals Mouse Borealin Antibody (OTI4G3) (NBP2-46199) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CDCA8 (NP_060571) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDCA8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Borealin Antibody (OTI4G3)

• BOR
• BOREALIN
• cell division cycle associated 8
• Cell division cycle-associated protein 8
• Dasra B
• DasraB
• dasra-B
• FLJ10468
• FLJ12042
• hDasra-B
• MESRGP
• PESCRG3
• Pluripotent embryonic stem cell-related gene 3 protein

Background

Borealin (CDCA8) is a component of the chromosomal passenger complex (CPC), which is required for stability of the bipolar mitotic spindle and acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA.

Borealin has been shown to interact with Aurora B and survivin (BIRC5). Knockdown of Borealin by small interfering RNA resulted in severe chromosome misalignment at metaphase and accumulation of multiple interphase nuclei. Borealin is also related to the proliferation of human embryonic stem (hES) cells and is highly expressed in mouse undifferentiated ES cells.
Borealin antibodies are useful tools for stem cell studies and cell division research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Borealin Antibody (NBP2-46199) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Jiang Danxian ICC Pig 01/02/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: pork liver
• Species: Pig
• Lot: W001

Bioinformatics

• Gene Symbol: CDCA8