Boll Antibody

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Immunohistochemistry-Paraffin: Boll Antibody [NB300-904] - (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Boll Antibody Summary

Immunogen
Peptide with sequence C-QPEPIKTVWSIHY corresponding to C-Terminus according to NP_932074.1, NP_149019.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BOLL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 22 kDa band in human testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6 kDa band according to NP_932074.1 and 31.3 kDa band according to NP_149019.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36 kDa band in human and mouse testisscientific literature (PMID: 11390979). IHC-P: Human testis shows cytoplasm staining in spermatocytes, but not in spermatogonia.
Positive Control
Boll Lysate (NBL1-08018)
Publications
Read Publications using NB300-904.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Boll Antibody

  • bol, boule-like (Drosophila)
  • BOULEbol (Drosophila boule homolog)-like
  • protein boule-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Boll Antibody (NB300-904)(2)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BOLL
Entrez
Uniprot