BOK Antibody

Western blot shows lysates of PC-3 human prostate cancer cell line, Hepa 1-6 mouse hepatoma cell line, and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BOK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BOK at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BOK Antibody Summary

• Immunogen: E. coli-derived recombinant human BOK
  Met15-Val123
  Accession # Q9UMX3
• Specificity: Detects human, mouse, and rat BOK in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BOK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BOK Antibody

• bcl2-L-9
• BCL2L9bcl-2-related ovarian killer protein
• Bcl-2-like protein 9
• BCL2-related ovarian killer
• BOK
• BOKL
• hBOK
• MGC4631

Background

BOK (Bcl-2-related ovarian killer protein; also Bcl2L9 and Mtd) is a 27 kDa member of the Bax subgroup of the Bcl-2 family of molecules. It is expressed in spermatocytes, ovarian granulosa cells, and liver cells. BOK is activated by E2F proteins, and sensitizes replicating cells to apoptosis when under stress. It is normally found in the cytosol, and upon activation, becomes incorporated into mitochondrial membranes. Human BOK is 212 amino acids (aa) in length and contains one BH4 domain (aa 32‑44), one BH3 domain (aa 66‑82), a BH1 domain (aa 112‑131) and a BH2 domain (aa 164‑178). A potential transmembrane span has been identified between aa 189‑210. BOK heterodimerizes with Mcl-1, BHRF-1, Bfl-1 and Bnip3, and is known to form heterooligomers. There are two potential splice variants. One contains an alternative start site at Met145, while another shows a five aa substitution for aa 117‑212. Over aa 15‑123, human BOK shares 94% aa identity with mouse BOK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BOK Antibody (AF6067)(1)

Publication using AF6067

• X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep, 2017-07-11;7(1):5081. 2017-07-11 [PMID: 28698547] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BOK
• Uniprot:
  • Q9UMX3(Human)