BMP-8 Antibody (158708) [Alexa Fluor® 532]

• Applications: WB
• Clone: 158708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

BMP-8 Antibody (158708) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human BMP‑8
  Ala264-His402
  Accession # AAP74559
• Specificity: Detects human BMP-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP-2, -3, -4, -5, -6, or -7 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2‑4). BMP‑8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP‑8a is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP‑8a shares 90% and 68% aa sequence identity with mouse mature BMP‑8a and -8b, respectively. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5‑7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10‑12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.