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BMP-6 Antibody (74219.11)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, B/N
Clone
74219.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

BMP-6 Antibody (74219.11) Summary

Immunogen
Recombinant full length protein (Human) expressed in CHO cells.
Localization
Secreted
Specificity
This shows less than 5 % cross-reactivity with recombinant human BMP5 and BMP7 and no cross-reactivity with recombinant human BMP2 and BMP4 under reducing conditions.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BMP6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: Use at a concentration of 0.5 - 1.0 ug/ml. The detecton limit for recombinant human BMP-6 is approximately 6.2 ng/well.Inhib: Use at a concentration of 1 - 3 ug/ml.WB: Use at a concentration of 1 - 20 ug/ml. The detection limit for recombinant human BMP6 is approximately 20 ng/lane under non-reducing conditions.Anti-Human Bone Morphogenetic Protein 6 (BMP6) is measured by its ability to neutralize the bioactivity of BMP6 in osteoblast MC3T3-E1 cells. Recombinant human BMP6 is added to various concentrations of the antibody for 1 hour at 37. This antibody has neutralizing activity at 0.5-2 ug/ml in an assay using ATDC-5 cells, which indicates that it has specificity to an epitope of the target which neutralizes its functional activity.
Control
BMP-6 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 5% Trehalose
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for BMP-6 Antibody (74219.11)

  • BMP6
  • BMP-6
  • bone morphogenetic protein 6
  • vegetal related growth factor (TGFB-related)
  • vegetal-related (TGFB related) cytokine
  • VG-1-R
  • VG-1-related protein
  • Vg1-related sequence
  • VGR
  • VGR1
  • Vgr-1

Background

Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BMP6
Entrez
Uniprot