Recombinant Human BMP-4 Protein

SDS-Page: Recombinant Human BMP-4 Protein [NBC1-19056] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human BMP-4 Protein Summary

• Description: An un-tagged recombinant protein corresponding to amino acids 293-408 of BMP4.
  
  

  Source: E.coli

  Amino Acid Sequence: MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BMP4
• Purity: >85%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 13.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM Sodium Citrate buffer (pH 3.5), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human BMP-4 Protein

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

Bone morphogenetic protein 4 (BMP4) is a member of the bone morphogenetic protein family which belongs to the TGF-beta superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP4 was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMP4