Mouse BMP-4 ELISA Kit (Chemiluminescence)

ELISA: Mouse BMP-4 ELISA Kit (Chemiluminescence) [NBP2-62851] - Samples were spiked with high concentrations of Mouse BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse BMP-4 ELISA Kit (Chemiluminescence) [NBP2-62851] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 12.50-800 pg/mL
• Sensitivity: 7.50 pg/mL

Mouse BMP-4 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Mouse BMP-4 in samples. No significant cross-reactivity or interference between Mouse BMP-4 and analogues was observed.
• Standard Curve Range: 12.50-800 pg/mL
• Sensitivity: 7.50 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 10.78%
• Intra-Assay: CV% < 10.78%
• Spike Recovery: 91-118%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: BMP4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse BMP-4 ELISA Kit (Chemiluminescence)

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for BMP-4 ELISA Kit (NBP2-62851). (Showing 1 - 2 of 2 FAQ).

1. I have some questions about Mouse BMP-4 ELISA Kit (Chemiluminescence). Is RIPA buffer compatible with the kit. I have samples that have been RIPA lysed and just wondering can I use this assay
   • RIPA is not recommended. Please see manual regarding tissue homogenates.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BMP4