BMP-3b/GDF-10 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

BMP-3b/GDF-10 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human BMP‑3b/GDF‑10
  Gln369-Arg478
  Accession # Q5VSQ8
• Specificity: Detects human BMP‑3b/GDF‑10 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) BMP-3 is observed and less than 2% cross-reactivity with rhBMP-2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-3b/GDF-10 Antibody [Alexa Fluor® 647]

• BIP
• BMP3b
• BMP-3b
• bone morphogenetic protein 3B
• Bone-inducing protein
• GDF10
• GDF-10
• growth differentiation factor 10
• Growth/differentiation factor 10

Background

BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta  family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2‑4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.