Human BMP-3 ELISA Kit (Colorimetric)

ELISA: Human BMP-3 ELISA Kit (Colorimetric) [NBP2-69993] - Samples were spiked with high concentrations of Human BMP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human BMP-3 ELISA Kit (Colorimetric) [NBP2-69993] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human BMP-3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human BMP-3 in samples. No significant cross-reactivity or interference between Human BMP-3 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6.07%
• Intra-Assay: CV% < 4.48%
• Spike Recovery: 89-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BMP3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human BMP-3 ELISA Kit (Colorimetric)

• BMP3
• BMP-3
• BMP3A
• BMP-3A
• bone morphogenetic protein 3 (osteogenic)
• bone morphogenetic protein 3
• Bone morphogenetic protein 3A
• bone morphogenetic protein-3
• Osteogenin

Background

Bone morphogenetic protein 3 (BMP3) is a 472 amino acid member of the bone morphogenetic protein (BMP) family. BMP3 negatively regulates bone density and is a member of the TGF beta superfamily. It is highly expressed in fracture tissue, predominantly in osteoblasts, osteoclasts and chondroblasts. It may play a role in otosclerosis, osteosarcoma, osteogenesis imperfecta, osteomalacia and periodontitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for BMP-3 ELISA Kit (NBP2-69993)(2)

Publications using NBP2-69993

• Venkatesan JK, Schmitt G, Speicher-Mentges S et al. Effects of rAAV-mediated overexpression of bone morphogenetic protein 3 (BMP-3) on the chondrogenic fate of human bone marrow-derived mesenchymal stromal cells Human gene therapy 2022-06-18 [PMID: 35722904] (ELISA)
• Shiwei Xie, Chenyang Zhao, Wei Chen, Gengwu Li, Zhiwei Xiong, Xiangjun Tang, Fan Zhang, Heng Xiao Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling International Journal of Biological Sciences 2021-01-01 [PMID: 34239363]

FAQs for BMP-3 ELISA Kit (NBP2-69993). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BMP3