Recombinant Human Blood Group B Transferase/GTB/ABO Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Blood Group B Transferase/GTB/ABO Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 54-354 of Human Blood Group B Transferase/GTB/ABO

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ABO
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human Blood Group B Transferase/GTB/ABO Protein

  • A transferase
  • A3GALNT
  • A3GALT1
  • ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase)
  • ABO glycosyltransferase
  • ABO
  • B transferase
  • B(A) alpha-1,3-galactosyltransferase
  • EC 2.4.1.37
  • EC 2.4.1.40
  • Fucosylglycoprotein 3-alpha-galactosyltransferase
  • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
  • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
  • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
  • GTB
  • Histo-blood group A transferase
  • histo-blood group A2 transferase
  • histo-blood group ABO system transferase
  • Histo-blood group B transferase
  • NAGAT

Background

ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ABO
Entrez