BLAME/SLAMF8 Antibody (250020)

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 250020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BLAME/SLAMF8 Antibody (250020) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
  Ala23-Asp233
  Accession # Q9P0V8
• Specificity: Detects recombinant human BLAME in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLAMF8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human BLAME/SLAMF8 Antibody (Catalog # MAB19073)
  ELISA Detection: Human BLAME/SLAMF8 Biotinylated Antibody (Catalog # BAM19074)
  Standard: Recombinant Human BLAME/SLAMF8 (Catalog # 1907-BL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLAME/SLAMF8 Antibody (250020)

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Background

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19% - 26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLAMF8
• Uniprot:
  • Q9P0V8()