285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog #
MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B)." class="big_lightbox">
285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog #
MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B)." alt="Human peripheral blood mononuclear cells were treated for with Recombinant Human IFN-gamma (Catalog #
285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog #
MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B)." class="big_thumb" />
285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." alt="Human peripheral blood mononuclear cells were treated for with Recombinant Human IFN-gamma (Catalog # 285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." class="big_thumb" />
HAF019). A specific band was detected for BLAME/SLAMF8 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected, transfected with EGFP, or transfected with human BLAME. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human BLAME/SLAMF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BLAME/SLAMF8 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
![Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with NBP2-26110 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26110 (1 ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-Western-Blot-NBP2-26110-img0001.jpg "Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with NBP2-26110 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26110 (1 ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-Immunohistochemistry-Paraffin-NBP2-26110-img0002.jpg)
285-IF) then stained with Human BLAME/SLAMF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1907, filled histogram) or control antibody (Catalog # ![Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-56595] - Staining in human lymph node and cerebral cortex tissues using anti-SLAMF8 antibody. Corresponding SLAMF8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-Immunohistochemistry-Paraffin-NBP2-56595-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-56595] - Staining in human lymph node and cerebral cortex tissues using anti-SLAMF8 antibody. Corresponding SLAMF8 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-56595] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-Immunohistochemistry-Paraffin-NBP2-56595-img0003.jpg)
![Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-56595] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-Immunohistochemistry-Paraffin-NBP2-56595-img0004.jpg)
![Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody (242) [NBP2-90657] - Staining of mouse SLAMF8 in mouse spleen (from 2 donors) with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/BLAME-SLAMF8-Antibody-242-Immunohistochemistry-Paraffin-NBP2-90657-img0001.jpg "Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody (242) [NBP2-90657] - Staining of mouse SLAMF8 in mouse spleen (from 2 donors) with rabbit monoclonal antibody (1:200).")
