Recombinant Human Bim His Protein

SDS-Page: Recombinant Human Bim Protein [NBP1-98873] - 15% SDS-PAGE (3 ug).

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Bim His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-138 of Human Bim
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAKQPS DVSSECDREG RQLQPAERPP QLRPGAPTSL QTEPQDRSPA PMSCDKSTQT PSPPCQAFNH YLSAMASMRQ AEPADMRPEI WIAQELRRIG DEFNAYYARR VFLNNYQAAE DHPRMVILRL LRYIVRLVWR MH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BCL2L11
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 18.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 2M UREA, 20% glycerol, 5mM DTT, 300 mM NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human Bim His Protein

• BAM
• bcl-2 interacting mediator of cell death
• bcl-2 interacting protein Bim
• Bcl2-interacting mediator of cell death
• bcl2-L-11
• BCL2-like 11 (apoptosis facilitator)
• bcl-2-like protein 11
• bcl-2-related ovarian death agonist
• BIM-alpha6
• BIM-beta6
• BIM-beta7
• BIMBOD
• BimEL
• BimL

Background

BCL2L11 (Bcl-2-like protein 11) belongs to the Bcl-2 family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of BCL2L11 can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Recombinant human BCL2L11 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Bim Recombinant Protein (NBP1-98873). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: BCL2L11