Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in control (vector only transfected HEK293T lysate) and BGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NBP1-84971] - Staining of human cell line BJ shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Analysis in human placenta and skeletal muscle tissues. Corresponding BGN RNA-seq data are presented for the same tissues.
Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human testis shows weak to moderate cytoplasmic positivity in peritubular myoid cells.
Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human kidney shows moderate extracellular space positivity.
Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: RGFWDFTLDDGPFMMNDEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSL
Specificity of human Biglycan antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Mouse (94%), Rat (93%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
FAQs for Biglycan Antibody (NBP1-84971). (Showing 1 - 1 of 1 FAQs).
I have a question regarding the Biglycan antibody NBP1-84971. It is stated that the species reactivity is human; is there a chance it will work in mice to, or do you known for a fact that the antibody does not label mouse tissues? I need a anti-biglycan antibody, for IHC (paraffin) on mouse tissue; can you recommend any of your antibodies for this purpose?
We have not yet tested this antibody on mouse, however the % homology is 95%, and our lab recommends use when the homology is 85% or above. If you would like to test this you could apply for our Innovators Reward Program. Unfortunately we do not have an anti Biglycan that has been validated for use in both mouse and IHC-P.
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