BID Antibody Pair [HRP]

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Sandwich ELISA: BID Antibody Pair [HRP] [NBP2-79468] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications S-ELISA
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Standard Curve Range
7.81-500 pg/ml
Sensitivity
7.8 pg/ml

Order Details

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Catalog# & Conjugate Size Price

BID Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human BID.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human BID.
Standard Curve Range
7.81-500 pg/ml
Sensitivity
7.8 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Mouse
Gene
BID

Applications/Dilutions

Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in CBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.25 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Standard: Each vial contains 17 ng of recombinant BID.
  2. Capture Antibody: 0.5 mg/ml of mouse anti-BID monoclonal antibody.
  3. Detection Antibody: 1.0 mg/ml mouse anti-BID monoclonal antibody conjugated to horseradish-peroxidase (HRP).

Alternate Names for BID Antibody Pair [HRP]

  • apoptic death agonist
  • BH3 interacting domain death agonist
  • BH3-interacting domain death agonist
  • BID isoform ES(1b)
  • BID isoform L(2)
  • BID isoform Si6
  • BID
  • desmocollin type 4
  • FP497
  • Human BID coding sequence
  • MGC15319
  • MGC42355
  • p22 BID
  • tbid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Other Available Formats

HRP NBP2-79468
HRP NBP2-79468

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Blogs on BID.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

active/cleaved Caspase 2 - Inducing apoptosis in response to cellular stress
Caspase-2 is a highly conserved member of the caspase family involved in the initiation and execution of apoptosis. While its function is still poorly understood, caspase-2 is thought to be important for apoptosis in response to DNA damage, bacteri...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

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Bioinformatics

Gene Symbol BID