BID Antibody (OTI1G10) - Azide and BSA Free

Images

 
Western Blot: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID.
Immunocytochemistry/ Immunofluorescence: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of HeLa cells.
Immunohistochemistry: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunohistochemistry: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BID Antibody (OTI1G10) - Azide and BSA Free [NBP2-70254] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

BID Antibody (OTI1G10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BID
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400-4000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:150
Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for BID Antibody (OTI1G10) - Azide and BSA Free

  • apoptic death agonist
  • BH3 interacting domain death agonist
  • BH3-interacting domain death agonist
  • BID isoform ES(1b)
  • BID isoform L(2)
  • BID isoform Si6
  • BID
  • desmocollin type 4
  • FP497
  • Human BID coding sequence
  • MGC15319
  • MGC42355
  • p22 BID
  • tbid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BID Antibody (NBP2-70254) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BID Antibody (NBP2-70254) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70254AF350
Alexa Fluor 405 NBP2-70254AF405
Alexa Fluor 488 NBP2-70254AF488
Alexa Fluor 532 NBP2-70254AF532
Alexa Fluor 594 NBP2-70254AF594
Alexa Fluor 647 NBP2-70254AF647
Alexa Fluor 700 NBP2-70254AF700
Alexa Fluor 750 NBP2-70254AF750
Biotin NBP2-70254B
DyLight 350 NBP2-70254UV
DyLight 405 NBP2-70254V
DyLight 488 NBP2-70254G
DyLight 550 NBP2-70254R
DyLight 594 NBP2-70254DL594
DyLight 650 NBP2-70254C
DyLight 680 NBP2-70254FR
DyLight 755 NBP2-70254IR
FITC NBP2-70254F
HRP NBP2-70254H
Janelia Fluor 549 NBP2-70254JF549
Janelia Fluor 646 NBP2-70254JF646

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Blogs on BID.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

active/cleaved Caspase 2 - Inducing apoptosis in response to cellular stress
Caspase-2 is a highly conserved member of the caspase family involved in the initiation and execution of apoptosis. While its function is still poorly understood, caspase-2 is thought to be important for apoptosis in response to DNA damage, bacteri...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

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Bioinformatics

Gene Symbol BID