BICD1 Antibody - BSA Free

Western Blot: BICD1 Antibody [NBP1-76922] - Daudi cell lysate with BICD1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: BICD1 Antibody - BSA Free [NBP1-76922] - Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: BICD1 Antibody - BSA Free [NBP1-76922] - Immunofluorescence of BICD1 in Daudi cells with BICD1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BICD1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BICD1 Antibody - BSA Free (NBP1-76922) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BICD1. The immunogen is located within the last 50 amino acids of BICD1. Amino Acid Squence: CPTVSPDTALPEEQPHS
• Specificity: At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BICD1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2.5-10 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  BICD1 Peptide (NBP1-76922PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BICD1 Antibody - BSA Free

• Bicaudal D (Drosophila) homolog 1
• bicaudal D homolog 1 (Drosophila)
• Bic-D 1
• BICD
• cytoskeleton-like bicaudal D protein homolog 1
• protein bicaudal D homolog 1

Background

BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells. At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BICD1
• Uniprot:
  • NP_001705(Human)