BICD1 Antibodies
We offer BICD1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each BICD1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BICD1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the BICD1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BICD1 polyclonal antibodies.
Alternate Names for BICD1 Antibodies
anti-BICD1 antibody, anti-Bic-D 1 antibody, anti-Bicaudal D (Drosophila) homolog 1 antibody, anti-bicaudal D homolog 1 (Drosophila) antibody, anti-BICD antibody, anti-cytoskeleton-like bicaudal D protein homolog 1 antibody, anti-protein bicaudal D homolog 1 antibody
8 Results for "bicd1" in Primary Antibodies
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Immunohistochemistry-Paraffin: BICD1 Antibody [NBP1-85843] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85843-img0009.jpg "Immunohistochemistry-Paraffin: BICD1 Antibody [NBP1-85843] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: BICD1 Antibody [NBP1-85843] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85843-img0006.jpg)
![Immunohistochemistry-Paraffin: BICD1 Antibody [NBP1-85843] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85843-img0007.jpg)
![Immunohistochemistry-Paraffin: BICD1 Antibody [NBP1-85843] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85843-img0008.jpg)
![Western Blot: BICD1 Antibody [NBP1-76922] - Daudi cell lysate with BICD1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-76922-img0001.jpg "Western Blot: BICD1 Antibody [NBP1-76922] - Daudi cell lysate with BICD1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: BICD1 Antibody - BSA Free [NBP1-76922] - Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76922_rabbit-polyclonal-bicd1-antibody-2711202420405518.jpg)
![Immunocytochemistry/ Immunofluorescence: BICD1 Antibody - BSA Free [NBP1-76922] - Immunofluorescence of BICD1 in Daudi cells with BICD1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76922_rabbit-polyclonal-bicd1-antibody-271120242035240.jpg)
![Western Blot: BICD1 Antibody [NBP3-10778] - Western blot analysis of BICD1 in Fetal Liver lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP3-10778-img0001.jpg "Western Blot: BICD1 Antibody [NBP3-10778] - Western blot analysis of BICD1 in Fetal Liver lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: BICD1 Antibody [NBP3-10679] - Western blot analysis of BICD1 in Human Thymus Tumor lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP3-10679-img0001.jpg "Western Blot: BICD1 Antibody [NBP3-10679] - Western blot analysis of BICD1 in Human Thymus Tumor lysates. Antibody dilution at 1ug/ml")
![Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunoprecipitation-NBP1-78734-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.")