beta Sarcoglycan Antibody (1C10) - Azide and BSA Free

Western Blot: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - SGCB monoclonal antibody (M02), clone 1C10 Analysis of SGCB expression in MCF-7.

Immunocytochemistry/Immunofluorescence: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - Analysis of monoclonal antibody to SGCB on MCF-7 cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - Analysis of monoclonal antibody to SGCB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

Sandwich ELISA: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - Detection limit for recombinant GST tagged SGCB is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

beta Sarcoglycan Antibody (1C10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SGCB (NP_000223, 87 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVK
• Specificity: SGCB (1C10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SGCB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta Sarcoglycan Antibody (1C10) - Azide and BSA Free

• 43 kDa dystrophin-associated glycoprotein
• 43DAG
• A3b
• A3bbeta-SG
• beta Sarcoglycan
• beta-Sarcoglycan
• beta-sarcoglycan(43kD dystrophin-associated glycoprotein)
• Beta-SG
• LGMD2E
• limb girdle muscular dystrophy 2E (non-linked families)
• Sarcoglycan beta
• sarcoglycan, beta (43kD dystrophin-associated glycoprotein)
• sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• SGC
• SGCB

Background

The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and provides structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. There are 3 main subcomplexes of the DGC: the cytoplasmic proteins dystrophin (DMD; MIM 300377) and syntrophin (SNTA1; MIM 601017), the alpha- and beta-dystroglycans (see MIM 128239), and the sarcoglycans (see, e.g., SGCA; MIM 600119) (Crosbie et al., 2000 [PubMed 10942431]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SGCB
• Entrez:
  • 6443(Human)
• OMIM:
  • 600900(Human)
• Uniprot:
  • NP_000223()