beta-Glucuronidase/GUSB Antibody [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 680

beta-Glucuronidase/GUSB Antibody [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human beta ‑Glucuronidase/GUSB in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Glucuronidase/GUSB Antibody [Alexa Fluor™ Plus 680]

• beta-D-glucuronidase
• Beta-G1
• beta-glucuronidase
• BG
• EC 3.2.1.31
• FLJ39445
• glucuronidase, beta
• GUSB
• MPS7

Background

Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).

1. Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
2. Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
3. Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
4. Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.