beta-Glucuronidase/GUSB Antibody [Unconjugated]

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human beta -Glucuronidase/GUSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -Glucuronidase/GUSB at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Glucuronidase/GUSB at approximately 92 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human beta ‑Glucuronidase/GUSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6144) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

beta-Glucuronidase/GUSB Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta ‑Glucuronidase/GUSB
  Met1-Thr651
  Accession # AAA52561
• Specificity: Detects human beta ‑Glucuronidase/GUSB in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GUSB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Glucuronidase/GUSB Antibody [Unconjugated]

• beta-D-glucuronidase
• Beta-G1
• beta-glucuronidase
• BG
• EC 3.2.1.31
• FLJ39445
• glucuronidase, beta
• GUSB
• MPS7

Background

Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).

1. Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
2. Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
3. Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
4. Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Glucuronidase/GUSB Antibody (AF6144)(2)

Publications using AF6144

• D Wang, Y Qiu, J Fan, Y Liu, W Chen, Z Li, W Chen, X Wang Upregulation of C/EBP Homologous Protein induced by ER Stress Mediates Epithelial to Myofibroblast Transformation in ADTKD-UMOD International Journal of Medical Sciences, 2022-01-24;19(2):364-376. 2022-01-24 [PMID: 35165522] (IHC, Human)
• Ward , S T, Li , K K, Hepburn , E, Weston , C J, Curbishley , S M, Reynolds , G M, Hejmadi , R K, Bicknell , R, Eksteen , B, Ismail , T, Rot , A, Adams , D H The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer, 2014-11-18;112(2):319-28. 2014-11-18 [PMID: 25405854] (Western Blot, Human)

Bioinformatics

• Gene Symbol: GUSB
• Uniprot:
  • AAA52561()