beta-Galactosidase-1/GLB1 Antibody

Images

 
Western Blot: beta-Galactosidase-1/GLB1 Antibody [NBP1-32018] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: beta-Galactosidase-1/GLB1 Antibody [NBP1-32018] - Paraffin-embedded human ovarian carcinoma. beta-Gal antibody [N2C3] dilution: 1:500.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml

Order Details

beta-Galactosidase-1/GLB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human beta-Gal. The exact sequence is proprietary.
Localization
Isoform 1: Lysosome, Isoform 2: Cytoplasm, perinuclear region
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Dog (82%), Cat (81%).

Alternate Names for beta-Galactosidase-1/GLB1 Antibody

  • Acid beta-galactosidase
  • beta-galactosidase
  • betaGalactosidase1
  • beta-Galactosidase-1/GLB1
  • EBP
  • EC 3.2.1.23
  • elastin receptor 1 (67kD)
  • Elastin receptor 1
  • elastin receptor 1, 67kDa
  • ELNR1
  • galactosidase, beta 1
  • GLB1
  • Lactase
  • MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol GLB1
Entrez
Uniprot

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