beta-Galactosidase-1/GLB1 Antibody

Western Blot: beta-Galactosidase-1/GLB1 Antibody [NBP1-32018] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Gal antibody [N2C3] diluted at 1:1000.

Immunohistochemistry-Paraffin: beta-Galactosidase-1/GLB1 Antibody [NBP1-32018] - Human ovarian carcinoma.beta-Gal antibody [N2C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

beta-Galactosidase-1/GLB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human beta-Gal. The exact sequence is proprietary.
• Localization: Isoform 1: Lysosome, Isoform 2: Cytoplasm, perinuclear region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (82%), Feline (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for beta-Galactosidase-1/GLB1 Antibody

• Acid beta-galactosidase
• beta-galactosidase
• betaGalactosidase1
• beta-Galactosidase-1
• EBP
• EC 3.2.1.23
• elastin receptor 1 (67kD)
• Elastin receptor 1
• elastin receptor 1, 67kDa
• ELNR1
• galactosidase, beta 1
• GLB1
• Lactase
• MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GLB1
• Entrez:
  • 2720(Human)
• Uniprot:
  • P16278(Human)