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beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free

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Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1 .
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for ...read more
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70829] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GLB1(NP_001073279) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GLB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
72.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for beta-Galactosidase-1/GLB1 Antibody (OTI1C9) - Azide and BSA Free

  • Acid beta-galactosidase
  • beta-galactosidase
  • betaGalactosidase1
  • beta-Galactosidase-1
  • EBP
  • EC 3.2.1.23
  • elastin receptor 1 (67kD)
  • Elastin receptor 1
  • elastin receptor 1, 67kDa
  • ELNR1
  • galactosidase, beta 1
  • GLB1
  • Lactase
  • MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GLB1