Beta-endorphin Antibody (B31.15) - BSA Free

Immunohistochemistry: Beta-endorphin Antibody (B31.15) [NB500-414] - Staining of human pituitary gland (frozen sections) with anti-human Beta-endorphin (B31.15).

• Reactivity: Hu
• Applications: IHC
• Clone: B31.15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Beta-endorphin Antibody (B31.15) - BSA Free Summary

• Description: Novus Biologicals Mouse Beta-endorphin Antibody (B31.15) - BSA Free (NB500-414) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human beta Endorphin (full length native protein).
• Specificity: The antibody B31.15 reacts with human beta Endorphin, an endogenous opiate derived from ACTH gene. ACTH (Corticotropin; human 39 aa) is synthesized by the anterior pituitary gland and stimulates the adrenal cortex; 6 hormones are derived from one ACTH gene: ACTH, lipotropin, alpha-MSH, beta-MSH, endorphin, and one other.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POMC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: Purity is > 95% (by SDS-PAGE). Immunohistochemistry-Paraffin sections: Staining technique: Standard ABC technique (DAB+) Pretreatment: Heat retrieval (microwave oven) Incubation: overnight at 4oC

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Beta-endorphin Antibody (B31.15) - BSA Free

• ACTH
• adrenocorticotropic hormone
• adrenocorticotropin
• alpha-melanocyte-stimulating hormone
• alpha-MSH
• beta-endorphin
• beta-LPH
• beta-melanocyte-stimulating hormone
• beta-MSH
• CLIP
• corticotropin-like intermediary peptide
• corticotropin-lipotropin
• gamma-LPH
• gamma-MSH
• lipotropin beta
• lipotropin gamma
• LPH
• melanotropin alpha
• melanotropin beta
• melanotropin gamma
• met-enkephalin
• MSH
• NPP
• POC
• pro-ACTH-endorphin
• proopiomelanocortin preproprotein
• proopiomelanocortin
• pro-opiomelanocortin

Background

Beta-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. Beta-endorphin is a peptide, 31 amino acids long, resulting from processing of the precursor pro-opiomelanocortin (POMC). beta-endorphin is found in neurons of the hypothalamus as well as the pituitary gland. It is an agonist of the opioid receptors, with evidence suggesting that it serves as the endogenous ligand of the u-opioid receptor, the same receptor to which the chemicals extracted from opium, such as morphine and codeine, have their analgesic and addictive effects

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Beta-endorphin Antibody (NB500-414). (Showing 1 - 1 of 1 FAQ).

1. Does the antibody (Cat# NB500-414) recognize other peptide hormones that are cleaved from POMC?
   • This antibody will detect full length POMC and also beta-endorphin, however we have not generated any testing data where we have used it against isolates of the other proteins which are cleaved from POMC. As such I cannot be certain whether or not it would recognise these.

Bioinformatics

• Gene Symbol: POMC
• Entrez:
  • 5443(Human)
• Uniprot:
  • P01189()