beta 2-Microglobulin Antibody (B2M/961)

Images

 
Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Immunofluorescence Analysis of HeLa cells labeling with beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse ...read more
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).
Flow Cytometry: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Flow Cytometric Analysis of Human HeLa cells. beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control ...read more
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Bladder Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Colon Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Melanoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Renal Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-P
Clone
B2M/961
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

beta 2-Microglobulin Antibody (B2M/961) Summary

Immunogen
Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769 )
Localization
Cell Surface and Extracellular (Secreted)
Marker
Renal Failure & Tumor Marker
Specificity
Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • Western Blot 0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and non-human primates.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47704)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for beta 2-Microglobulin Antibody (B2M/961)

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol B2M
Uniprot