BCMA/TNFRSF17 Antibody (1004023) [Unconjugated]

RPMI8226 human myeloma cell line was stained with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB1931, filled histogram) or isotype control antibody (Catalog # MAB10041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

BCMA/TNFRSF17 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB1931) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 1004023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BCMA/TNFRSF17 Antibody (1004023) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCMA /TNFRSF17
  Met1-Ala54
  Accession # Q02223
• Specificity: Detects human BCMA/TNFRSF17 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCMA/TNFRSF17 Antibody (1004023) [Unconjugated]

• B cell maturation antigen
• B-cell maturation protein
• BCMA
• BCMAtumor necrosis factor receptor superfamily member 17
• BCMB-cell maturation factor
• CD269 antigen
• CD269
• TNFRSF13A
• TNFRSF17
• tumor necrosis factor receptor superfamily, member 17

Background

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q02223()