BCL9-2 Antibody - BSA Free

Western Blot: BCL9-2 Antibody [NBP1-76542] - HeLa cell lysate at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: BCL9-2 Antibody - BSA Free [NBP1-76542] - Immunofluorescence of BCL9-2 in human breast tissue with BCL9-2 antibody at 20 ug/ml.Green: BCL9-2 Antibody Blue: DAPI staining

Immunohistochemistry: BCL9-2 Antibody [NBP1-76542] - Staining of Bcl9L in human breast tissue with Bcl9L antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: BCL9-2 Antibody - BSA Free [NBP1-76542] - Immunocytochemistry of BCL9-2 in HeLa cells with BCL9-2 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BCL9-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCL9-2 Antibody - BSA Free (NBP1-76542) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human Bcl9L. The immunogen is located within amino acids 20 - 70 of Bcl9L. Amino Acid Squence: PAKPMHPENKLTNHGKTGNG
• Specificity: Bcl9L antibody is predicted to not cross-react with other Bcl family members. At least four isoforms of Bcl9L are known to exist; this antibody will detect all four.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL9L
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 ug/ml
• Theoretical MW: 195 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

• Control Peptide:

  BCL9-2 Peptide (NBP1-76542PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BCL9-2 Antibody - BSA Free

• B-cell CLL/lymphoma 9-like protein
• B-cell CLL/lymphoma 9-like
• B-cell lymphoma 9-like protein
• Bcl92
• Bcl9-2
• BCL9-like protein
• DLNB11
• DLNB11BCL9-2
• nuclear co-factor of beta-catenin signalling
• Protein BCL9-2

Background

Bcl9L, a homolog of Bcl9, was initially identified through a bioinformatics screening. It is expressed in fetal brain, adult lung, eye and prostate, in addition to several types of tumors including pancreatic and prostate cancers. Bcl9L has been shown to interact with beta-catenin, a target of the Wnt signaling pathway, and is required for enhanced beta-catenin-T-cell factor (TCF)-mediated transcription in colorectal tumor cells, possibly by translocating beta-catenin to the nucleus. Other studies have indicated that Bcl9L expression correlates with high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers, suggesting that activity may occur in other types of cancer. Bcl9L has also been shown to be critical for Wnt-mediate regulation of stem cell traits in colon epithelium and adenocarcinomas which are associated with tumor invasion, metastasis, and resistance to therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL9L
• Entrez:
  • 283149(Human)
  • 80288(Mouse)
• Uniprot:
  • Q86UU0()