BCL-W/BCL2L2 Overexpression Lysate

Images

 
Western Blot: Bcl2L2 Overexpression Lysate (Adult Normal) [NBL1-07950] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bcl2L2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BCL-W/BCL2L2 Overexpression Lysate Summary

Description
Transient overexpression lysate of BCL2-like 2 (BCL2L2) The lysate was created in HEK293T cells, using Plasmid ID RC211152 and based on accession number NM_004050. The protein contains a C-MYC/DDK Tag.
Gene
BCL2L2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
20.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BCL-W/BCL2L2 Overexpression Lysate

  • Apoptosis regulator Bcl-W
  • BCL2L2
  • Bcl2-L-2
  • BCL2-like 2
  • Bclw
  • Bcl-w
  • BCLWbcl-2-like protein 2
  • KIAA0271BCL2-L-2

Background

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BCL-W/BCL2L2 Lysate (NBL1-07950) (0)

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Other Available Formats

NBL1-07950

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Bioinformatics

Gene Symbol BCL2L2