Bcl G Antibody - BSA Free

Western Blot: Bcl G Antibody [NBP1-77223] - U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Bcl G Antibody - BSA Free [NBP1-77223] - Immunofluorescence of Bcl G in Mouse Testis cells with Bcl G antibody at 10 u/mL.

Immunohistochemistry: Bcl G Antibody - BSA Free [NBP1-77223] - Immunohistochemical staining of mouse testis tissue using Bcl G antibody at 2 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bcl G Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bcl G Antibody - BSA Free (NBP1-77223) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G. Amino Acid Squence: CDLEEIPLDDDDLNT
• Specificity: Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2L14
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 2.5-5 ug/ml
• Control Peptide:

  Bcl G Peptide (NBP1-77223PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for Bcl G Antibody - BSA Free

• apoptosis facilitator Bcl-2-like protein 14
• Apoptosis regulator Bcl-G
• Bcl2-L-14
• BCL2-like 14 (apoptosis facilitator)
• BCL-G
• BCLGbcl2-L-14

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L14
• Uniprot:
  • NM_030766(Human)