Bcl G Antibody (S06-3D8)

Western Blot: Bcl G Antibody (S06-3D8) [NBP3-19984] - Western blot detection of Bcl G in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19984 (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 43kDa.

Immunocytochemistry/Immunofluorescence: Bcl G Antibody (S06-3D8) [NBP3-19984] - Immunocytochemistry of Bcl G (green) in hela using NBP3-19984 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S06-3D8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Bcl G Antibody (S06-3D8) Summary

• Description: Novus Biologicals Rabbit Bcl G Antibody (S06-3D8) (NBP3-19984) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human Bcl G (Uniprot # Q9BZR8)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCL2L14
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Western Blot 1/1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Bcl G Antibody (S06-3D8)

• apoptosis facilitator Bcl-2-like protein 14
• Apoptosis regulator Bcl-G
• Bcl2-L-14
• BCL2-like 14 (apoptosis facilitator)
• BCL-G
• BCLGbcl2-L-14

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L14