BATF Antibody (687706) [Unconjugated]

Western blot shows lysates of KARPAS 299 human anaplastic large cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BATF at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 687706
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BATF Antibody (687706) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human BATF
  Met1-Pro125
  Accession # Q16520
• Specificity: Detects human BATF in ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BATF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BATF Antibody (687706) [Unconjugated]

• basic leucine zipper transcription factor, ATF-like
• BATF
• BATF1basic leucine zipper transcriptional factor ATF-like
• B-ATFSFA2
• B-cell-activating transcription factor
• SFA2
• SFA-2activating transcription factor B
• SF-HT-activated gene 2 protein

Background

BATF is an approximately 20 kDa member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser43. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8⁺ T cells, and CD8⁺ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BATF Antibody (MAB8054)(1)

Publication using MAB8054

• U Govender, B Corre, Y Bourdache, S Pellegrini, F Michel Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors J. Leukoc. Biol, 2017-02-27;0(0):. 2017-02-27 [PMID: 28242623] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BATF
• Uniprot:
  • Q16520()