BATF Antibody (687706) [Allophycocyanin]

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF APC-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8054A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 687706
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

BATF Antibody (687706) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human BATF
  Met1-Ser125
  Accession # Q16520
• Specificity: Detects Human BATF in ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BATF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BATF Antibody (687706) [Allophycocyanin]

• basic leucine zipper transcription factor, ATF-like
• BATF
• BATF1basic leucine zipper transcriptional factor ATF-like
• B-ATFSFA2
• B-cell-activating transcription factor
• SFA2
• SFA-2activating transcription factor B
• SF-HT-activated gene 2 protein

Background

BATF is a member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser⁴³. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8+ T cells, and CD8+ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BATF
• Uniprot:
  • Q16520()