BASC4 Antibody

Images

 
Western Blot: BASC4 Antibody [NBP1-77126] - 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: BASC4 Antibody [NBP1-77126] - Immunofluorescence of BCAS4 in human breast carcinoma tissue with BCAS4 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: BASC4 Antibody [NBP1-77126] - Human breast carcinoma with BCAS4 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BASC4 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4. The immunogen is located within the first 50 amino acids of BCAS4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 20 ug/ml
Control Peptide
BASC4 Peptide (NBP1-77126PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BASC4 Antibody

  • BCAS4/BCAS3 fusion
  • breast carcinoma amplified sequence 4
  • breast carcinoma amplified sequence 4/3 fusion protein
  • breast carcinoma-amplified sequence 4
  • CNOL
  • FLJ20495

Background

The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional BASC4 Products

BASC4 NBP1-77126

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Bioinformatics

Gene Symbol BCAS4
Uniprot