BASC4 Antibody - BSA Free

Images

 
Western Blot: BASC4 Antibody [NBP1-77126] - 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: BASC4 Antibody - BSA Free [NBP1-77126] - Immunohistochemistry of BASC4 in human breast carcinoma with BASC4 antibody at 5 u/mL.
Immunocytochemistry/ Immunofluorescence: BASC4 Antibody - BSA Free [NBP1-77126] - Immunofluorescence of BASC4 in human breast carcinoma tissue with BASC4 antibody at 20 u/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

BASC4 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit BASC4 Antibody - BSA Free (NBP1-77126) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4. The immunogen is located within the first 50 amino acids of BCAS4. Amino Acid Squence: DADQPEPMRSGARE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS4
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
Control Peptide
BASC4 Peptide (NBP1-77126PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for BASC4 Antibody - BSA Free

  • BCAS4/BCAS3 fusion
  • breast carcinoma amplified sequence 4
  • breast carcinoma amplified sequence 4/3 fusion protein
  • breast carcinoma-amplified sequence 4
  • CNOL
  • FLJ20495

Background

The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional BASC4 Products

Array NBP1-77126

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Bioinformatics

Gene Symbol BCAS4
Uniprot