DPM1 Antibody

Western Blot: DPM1 Antibody [NBP1-05034] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DPM1 Antibody [NBP1-05034] - (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DPM1 Antibody Summary

• Description: Novus Biologicals Goat DPM1 Antibody (NBP1-05034) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DPM1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RELEVRSPRQNKYS-C corresponding to N-Terminus according to NP_003850.1.
• Epitope: N Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: Western blot: Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.6kDa according to NP_003850.1). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Tonsil.).
• Control:

  DPM1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPM1 Antibody

• CDGIEEC 2.4.1.83
• dolichol monophosphate mannose synthase
• dolichol-phosphate mannosyltransferase
• Dolichyl-phosphate beta-D-mannosyltransferase
• dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
• DPM synthase
• Mannose-P-dolichol synthase
• MPD synthase
• MPDSDolichol-phosphate mannose synthase

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPM1 Antibody (NBP1-05034)(1)

Publication using NBP1-05034

• Ashida H, Maeda Y, Kinoshita T. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3 J Biol Chem 2006-01-13 [PMID: 16280320]

Bioinformatics

• Gene Symbol: DPM1
• Entrez:
  • 8813(Human)
• Uniprot:
  • NP_003850.1(Human)