BAF53A Antibodies
We offer BAF53A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each BAF53A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BAF53A Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the BAF53A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BAF53A polyclonal antibodies and browse our BAF53A monoclonal antibody catalog.
Alternate Names for BAF53A Antibodies
anti-BAF53A antibody, anti-ACTL6A antibody, anti-actin-like 6A antibody, anti-actin-like protein 6A antibody, anti-actin-related protein 4,53 kDa BRG1-associated factor A antibody, anti-Actin-related protein Baf53a antibody, anti-Actl6 antibody, anti-Arp4 antibody, anti-arpNbeta antibody, anti-BAF complex 53 kDa subunit antibody, anti-BAF53AACTL6 antibody, anti-BAF53MGC5382 antibody, anti-BRG1-associated factor 53A antibody, anti-hArpN beta antibody, anti-INO80 complex subunit K antibody, anti-INO80KARPN-BETA antibody
10 Results for "baf53a" in Primary Antibodies
![Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Simple-Western-NB100-61628-img0007.jpg "Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of three biological replicates. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30506#content) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0038.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0035.jpg)
![Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0036.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0034.jpg)
![Western Blot: BAF53A Antibody [NB100-61628] - BRD9 defines a distinct, non-canonical BAF complex. a Immunoprecipitation (IP)-mass spectrometry using BRD9 or IgG antibody from mouse ESCs. The plot shows the spectral count fold change in BRD9 IP relative to IgG & the corresponding AC test p values, calculated with two technical replicates using PatternLab. In orange are proteins that satisfy FC 2, AC test p < 0.05. b Immunoblotting analysis of fractions after mouse ESC nuclear lysates were subjected to a density sedimentation assay in 10–30% glycerol gradient. LMW & HMW indicate lower & higher molecular weights, respectively. Nonspecific bands are marked with an asterisk. Molecular weights from ladder are indicated. c Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, & BAF47. Blots developed using chemiluminescence are marked with double asterisks; each IP was taken from a different exposure. d Depletion IP experiment from ESCs using antibodies against BRG1 or BAF155. Each lane shows the remaining proteins after each successive IP, labeled 1 through 4. e IP experiment from ESCs using antibodies against IgG or BRD9, which was incubated in increasing concentration of urea. f Schematic of the esBAF, GBAF, & PBAF complexes in mouse ESCs. Colored in orange, pink, & mustard yellow are subunits that define each individual complex. In green is the enzymatic component, BRG1. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61628_rabbit-polyclonal-baf53a-antibody-310202415532068.jpg)
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0002.jpg "Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0004.jpg)
![Flow Cytometry: BAF53A Antibody [NB100-1421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Flow-Cytometry-NB100-1421-img0005.jpg)
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0003.jpg)
![Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Western-Blot-NBP3-16659-img0005.jpg "Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded mouse kidney using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0004.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded rat brain using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0001.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human appendix using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0002.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0003.jpg)
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Human placenta using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411353062.jpg "Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Human placenta using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-101220241136084.jpg)
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411433437.jpg)
![Western Blot: BAF53A Antibody [NBP3-38142] - Western blot analysis of various lysates using BAF53A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411392957.jpg)
![Western Blot: BAF53A Antibody [NBP2-55376] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP2-55376-img0002.jpg "Western Blot: BAF53A Antibody [NBP2-55376] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-55376] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55376-img0001.jpg)
![Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP2-99367-img0003.jpg "Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-99367] - Immunofluorescence staining of BAF53A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BAF53A polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99367-img0001.jpg)
![Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP1-55378-img0003.jpg "Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: BAF53A Antibody [NBP1-55378] - Mouse WT brain and Rat brain, concentration 2ug/ml.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP1-55378-img0002.jpg)
![Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)](http://images.novusbio.com/fullsize/BAF53A-Antibody-S03-1G4-Western-Blot-NBP3-19983-img0001.jpg "Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)")
![Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BAF53A-Antibody-3C4-Western-Blot-H00000086-M10-img0002.jpg "Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.")
![ELISA: BAF53A Antibody (3C4) [H00000086-M10] - Detection limit for recombinant GST tagged ACTL6A is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BAF53A-Antibody-3C4-ELISA-H00000086-M10-img0001.jpg)
![Western Blot: BAF53A Antibody (3E4.) [H00000086-M14] - Detection against Immunogen (68.31 KDa) .](http://images.novusbio.com/fullsize/BAF53A-Antibody-3E4-Western-Blot-H00000086-M14-img0001.jpg "Western Blot: BAF53A Antibody (3E4.) [H00000086-M14] - Detection against Immunogen (68.31 KDa) .")