BAF180/PB1 Antibody (CL0331) - Azide and BSA Free

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Analysis in human endometrium and skeletal muscle tissues using NBP3-43955 antibody. Corresponding BAF180/PB1 RNA-seq data are presented for the same tissues.

Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.

Staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.

Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-BAF180/PB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0331
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

BAF180/PB1 Antibody (CL0331) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse BAF180/PB1 Antibody (CL0331) - Azide and BSA Free (NBP2-30673) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q86U86, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PBRM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for BAF180/PB1 Antibody (CL0331) - Azide and BSA Free

• BAF180
• BAF180/PB1
• BAF180PB1BRG1-associated factor 180
• hPB1
• MGC156155
• MGC156156
• PB1
• PBRM1
• polybromo 1
• Polybromo-1D
• protein polybromo-1

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBRM1