BAF180/PB1 Antibody (CL0331)

Images

 
Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Orthogonal Strategies: Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the ...read more
Genetic Strategies: Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative ...read more
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clone
CL0331
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BAF180/PB1 Antibody (CL0331) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHA
Specificity
Specificity of human BAF180/PB1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PBRM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
BAF180/PB1 Recombinant Protein Antigen (NBP2-30673PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for BAF180/PB1 Antibody (CL0331)

  • BAF180PB1BRG1-associated factor 180
  • hPB1
  • MGC156155
  • MGC156156
  • polybromo 1
  • polybromo-1D
  • protein polybromo-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PBRM1
Uniprot