Bad Antibody

Product Discontinued

Bad Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 112-163 of Human Bad.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of BAD (R149) pAb in extracts from 293 cells treated with forskolin.
  Immunohistochemistry (IHC) analyzes of BAD (R149) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Bad Overexpression Lysate

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bad Antibody

• Bad
• BBC2
• BBC6
• bcl2 antagonist of cell death
• BCL2-antagonist of cell death protein
• BCL2-associated agonist of cell death
• Bcl-2-binding component 6
• BCL2-binding component 6
• BCL2-binding protein
• BCL2L8
• Bcl2-L-8
• BCL2L8bcl2-L-8
• Bcl-2-like protein 8
• BCL-X/BCL-2 binding protein
• Bcl-XL/Bcl-2-associated death promoter

Background

The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2, limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2, and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes, thereby causing restoration of Bax-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bad Antibody (NB100-91672)(1)

Publication using NB100-91672

• Mahalingam D, Espitia CM, Medina EC et al. Targeting PIM kinase enhances the activity of sunitinib in renal cell carcinoma. British Journal of Cancer. 2011-10-20 [PMID: 22015557] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: BAD
• Entrez:
  • 572(Human)
  • 64639(Rat)