Bad Antibody (64103) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 64103
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Bad Antibody (64103) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse Bad
• Specificity: Detects endogenous mouse Bad in Western blots. In Western blots, this antibody does not cross-react with human Bad. The antibody also detects a 32 kDa band in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bad Antibody (64103) [Alexa Fluor® 532]

• Bad
• BBC2
• BBC6
• bcl2 antagonist of cell death
• BCL2-antagonist of cell death protein
• BCL2-associated agonist of cell death
• Bcl-2-binding component 6
• BCL2-binding component 6
• BCL2-binding protein
• BCL2L8
• Bcl2-L-8
• BCL2L8bcl2-L-8
• Bcl-2-like protein 8
• BCL-X/BCL-2 binding protein
• Bcl-XL/Bcl-2-associated death promoter

Background

Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.