B7-H4 Antibody (2582H) [Unconjugated]

Western blot shows lysates of SK-BR-3 human breast cancer cell line, mouse uterus tissue, and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat B7-H4 Monoclonal Antibody (Catalog # MAB21542) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for B7-H4 at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB21542) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

B7-H4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse B7-H4 Monoclonal Antibody (Catalog # MAB21542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of SK‑BR‑3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for B7‑H4 at approximately 128 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat B7‑H4 Monoclonal Antibody (Catalog # MAB21542) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, CyTOF-ready, ICC/IF
• Clone: 2582H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

B7-H4 Antibody (2582H) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
  Phe29-Pro258
  Accession # Q7TSP5
• Specificity: Detects mouse B7-H4 in direct ELISAs. Detects human, mouse, and rat B7-H4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H4 Antibody (2582H) [Unconjugated]

• B7h.5
• B7H4
• B7-H4
• B7H4T-cell costimulatory molecule B7x
• B7S1
• B7S1VCTN1
• B7x
• B7XPRO1291
• FLJ22418
• Immune costimulatory protein B7-H4
• Protein B7S1
• T cell costimulatory molecule B7x
• V-set domain containing T cell activation inhibitor 1
• V-set domain-containing T-cell activation inhibitor 1
• Vtcn1

Background

B7-H4, also known as B7x and B7S1, belongs to the B7 family of costimulatory proteins and contains two extracellular Ig-like domains. B7-H4 is expressed on T cells, B cells, monocytes/macrophages, dendritic cells, and some tumor cells. B7-H4 signaling inhibits T cell activation, proliferation and maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q7TSP5()