B7-2/CD86 Antibody (37301) [Phycoerythrin]

Images

 
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human B7‑2/CD86 PE‑conjugated Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
37301
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

B7-2/CD86 Antibody (37301) [Phycoerythrin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86
Specificity
Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD86
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
FAB141P in the following application:

Publications
Read Publications using
FAB141P in the following applications:

  • 5 publications

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from ascites

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-2/CD86 Antibody (37301) [Phycoerythrin]

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon-gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-2/CD86 Antibody (FAB141P)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


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Review for B7-2/CD86 Antibody (FAB141P) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using FAB141P:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/21/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22517920
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 22517920

Details

Detection NotesSee PMID: 22517920

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 22517920

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FAQs for B7-2/CD86 Antibody (FAB141P) (0)

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Secondary Antibodies

 

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Additional B7-2/CD86 Products

Bioinformatics Tool for B7-2/CD86 Antibody (FAB141P)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (FAB141P). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-2/CD86 Antibody (FAB141P)

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Pathways for B7-2/CD86 Antibody (FAB141P)

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PTMs for B7-2/CD86 Antibody (FAB141P)

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/21/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD86
Entrez