B7-2/CD86 Antibody (1036438) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Clone: 1036438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B7-2/CD86 Antibody (1036438) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human B7-2/CD86
  Leu26-Pro247
  Accession # NP_787058
• Specificity: Detects human B7-2/CD86 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-2/CD86 Antibody (1036438) [Unconjugated]

• Activation B7-2 antigen
• B70
• B7-2 antigen
• B72
• B7-2
• B-lymphocyte activation antigen B7-2
• BU63
• CD28 antigen ligand 2
• CD28LG2B7-2 antigen)
• CD86 antigen
• CD86 molecule
• CD86
• CTLA-4 counter-receptor B7.2
• FUN-1
• LAB72
• MGC34413
• T-lymphocyte activation antigen CD86

Background

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature human B7-2 consists of a 224 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic tail (3, 4). Within the ECD, human B7-2 shares 59% aa sequence identity with mouse and rat B7-2. Alternative splicing of human B7-2 generates additional isoforms that lack both Ig-like domains or a region that includes the transmembrane segment. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3-5, 7, 9-11). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up-regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3-5, 7, 9, 10). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (12). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (10). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (13). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (14). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (15).

1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
3. Freeman, G.J. et al. (1993) Science 262:909.
4. Azuma, M. et al. (1993) Nature 366:76.
5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
6. Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
7. Hathcock, K.S. et al. (1993) Science 262:905.
8. Seino, K. et al. (1995) Int. Immunol. 7:1331.
9. Chen, C. et al. (1994) J. Immunol. 152:4929.
10. Lanier, L.L. et al. (1995) J. Immunol. 154:97.
11. Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
12. Wing, K. et al. (2011) Trends Immunol. 32:428.
13. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
14. Kuchroo, V.K. et al. (1995) Cell 80:707.
15. Short, J.J. et al. (2006) Virus Res. 122:144.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_787058()