B7-1/CD80 Antibody [Alexa Fluor® 532]

• Reactivity: Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

B7-1/CD80 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat B7‑1/CD80
  Ile39-Gln248
  Accession # AAB60503
• Specificity: Detects rat B7‑1/CD80 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) B7-1 is observed, 5% cross-reactivity with recombinant human B7-1 is observed, and less than 1%
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-1/CD80 Antibody [Alexa Fluor® 532]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

It is widely accepted that T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3 and PD-L2 (1). B7-1 is expressed on activated B cells, activated T cells, mature dendritic cells and macrophages (2). T cells express two different receptors capable of binding B7-1, CD28 and CTLA-4. CTLA-4 binds to B7-1 with a 20‑100 fold higher affinity than CD28. Engagement of CD28 by B7-1 increases T cell proliferation and IL-2 production and enhances the immune response (3). In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response (4). Rat B7-1 is a 320 amino acid (aa) protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Rat and human B7-1 share 47% amino acid identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.