B220/CD45R Antibody (20.96)


Flow Cytometry: CD45R Antibody (20.96) [NB100-65367] - Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD45R: RPE

Product Details

Reactivity ShSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
1 mg/ml

B220/CD45R Antibody (20.96) Summary

Ovine thymocytes
NB100-65367 recognizes the ovine CD45R cell surface antigen, a 220kD glycoprotein, which is expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas. *This clone has been reported to react with paraffin-embedded material following alcohol fixation. Please refer to ref. Sainte Marie, et al for alcohol fixation protocol.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline pH7.4
0.09% Sodium Azide
1 mg/ml
Protein G purified


  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Sheep.

Alternate Names for B220/CD45R Antibody (20.96)

  • B220
  • CD45 antigen
  • CD45R
  • EC
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Func, In vitro, In vivo, Flow-CS
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, Flow, IHC
Species: Sh
Applications: Flow, IHC, IHC-Fr, IP

Publications for B220/CD45R Antibody (NB100-65367) (0)

There are no publications for B220/CD45R Antibody (NB100-65367).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B220/CD45R Antibody (NB100-65367) (0)

There are no reviews for B220/CD45R Antibody (NB100-65367). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for B220/CD45R Antibody (NB100-65367) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-65367AF405
Alexa Fluor (R) 488 Labeled NB100-65367AF488
Alexa Fluor (R) 647 Labeled NB100-65367AF647
Alexa Fluor (R) 700 Labeled NB100-65367AF700
Allophycocyanin Labeled NB100-65367APC
DyLight 350 Labeled NB100-65367UV
DyLight 405 Labeled NB100-65367V
DyLight 405LS Labeled NB100-65367V3
DyLight 488 Labeled NB100-65367G
DyLight 680 Labeled NB100-65367FR
DyLight 755 Labeled NB100-65367IR
PE Labeled NB100-65367PE
PerCP Labeled NB100-65367PCP

Additional B220/CD45R Antibody (20.96) Products

B220/CD45R NB100-65367

Related Products by Gene

Bioinformatics Tool for B220/CD45R Antibody (NB100-65367)

Discover related pathways, diseases and genes to B220/CD45R Antibody (NB100-65367). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for B220/CD45R Antibody (NB100-65367)

Discover more about diseases related to B220/CD45R Antibody (NB100-65367).

Pathways for B220/CD45R Antibody (NB100-65367)

View related products by pathway.

PTMs for B220/CD45R Antibody (NB100-65367)

Learn more about PTMs related to B220/CD45R Antibody (NB100-65367).

Research Areas for B220/CD45R Antibody (NB100-65367)

Find related products by research area.

Blogs on B220/CD45R

There are no specific blogs for B220/CD45R, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol PTPRC

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-65367 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought