B220/CD45R Antibody (20.96)

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Flow Cytometry: B220/CD45R Antibody (20.96) [NB100-65367] - Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD45R: RPE

Product Details

Summary
Reactivity ShSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
20.96
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

B220/CD45R Antibody (20.96) Summary

Immunogen
Ovine thymocytes
Specificity
NB100-65367 recognizes the ovine CD45R cell surface antigen, a 220kD glycoprotein, which is expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas. *This clone has been reported to react with paraffin-embedded material following alcohol fixation. Please refer to ref. Sainte Marie, et al for alcohol fixation protocol.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for B220/CD45R Antibody (20.96)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65367AF405
Alexa Fluor 488 Labeled NB100-65367AF488
Alexa Fluor 647 Labeled NB100-65367AF647
Alexa Fluor 700 Labeled NB100-65367AF700
Allophycocyanin Labeled NB100-65367APC
DyLight 350 Labeled NB100-65367UV
DyLight 405 Labeled NB100-65367V
DyLight 488 Labeled NB100-65367G
DyLight 680 Labeled NB100-65367FR
DyLight 755 Labeled NB100-65367IR
PE Labeled NB100-65367PE
PerCP Labeled NB100-65367PCP

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B220/CD45R NB100-65367

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Bioinformatics

Gene Symbol PTPRC
Entrez